ArticleThe Plant cell2011
Medicago truncatula CYP716A12 is a multifunctional oxidase involved in the biosynthesis of hemolytic saponins.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 234 citations in OpenAlex.
- Oleanolic acid in the fight against cancer: a multifaceted natural strategy for modern oncology.Molecular diversity · 2026Review
- Disentangling direct and pleiotropic SNP effects in alfalfa (Medicago sativa L.) using causal graph learning.Scientific reports · 2026Article
- Metabolomics combined with transcriptomics analysis on ginsenosides accumulation in root of American ginseng plants under foliar applications of brassinolide.Frontiers in plant science · 2026Article
- Programmable saponin biosynthesis from gene networks to predictive biomanufacturing.Frontiers in plant science · 2026Review
- Transcriptome and Metabolome Analyses Reveal Differences in the Accumulation of Key Metabolites in Various Tissues ofMetabolites · 2025Article
- Metabolic and genetic analysis links TRITERPENE SYNTHASE 12 to oleanolic acid biosynthesis in grape berry wax.Journal of experimental botany · 2025Article
- Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins.Plant biotechnology journal · 2025Article
- A molecular representation system with a common reference frame for analyzing triterpenoid structural diversity.Plant communications · 2025Article
- Harnessing Apple Cell Suspension Cultures in Bioreactors for Triterpene Production: Transcriptomic Insights into Biomass and Triterpene Biosynthesis.International journal of molecular sciences · 2025Article
- Deep transcriptome and metabolome analysis to dissect untapped spatial dynamics of specialized metabolism in Saussurea costus (Falc.) Lipsch.Functional & integrative genomics · 2025Article
- Genome-wide identification and functional analysis of CYP450 genes in eggplant (Solanum melongena L.) with a focus on anthocyanin accumlation.BMC genomics · 2024Article
- Roles of three cytochrome P450 monooxygenases in triterpene biosynthesis and their potential impact on growth and development.Plant physiology · 2024Article
- Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltratedPlant biotechnology (Tokyo, Japan) · 2024Article
- Genome-Wide Identification of the CYP716 Gene Family inPlants (Basel, Switzerland) · 2024Article
- Cardiac glycosides protect wormseed wallflower (Erysimum cheiranthoides) against some, but not all, glucosinolate-adapted herbivores.The New phytologist · 2024Article
- Article
- Comparative transcriptomic and proteomic analyses of two salt-tolerant alfalfa (Frontiers in plant science · 2024Article
- Cardiac glycosides protect wormseed wallflower (bioRxiv : the preprint server for biology · 2023Article
- Comparative genomics of the medicinal plants Lonicera macranthoides and L. japonica provides insight into genus genome evolution and hederagenin-based saponin biosynthesis.Plant biotechnology journal · 2023Article
- Combinatorial optimization and spatial remodeling of CYPs to control product profile.Metabolic engineering · 2023Article
33 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Saponins, a group of glycosidic compounds present in several plant species, have aglycone moieties that are formed using triterpenoid or steroidal skeletons. In spite of their importance as antimicrobial compounds and their possible benefits for human health, knowledge of the genetic control of saponin biosynthesis is still poorly understood. In the Medicago genus, the hemolytic activity of saponins is related to the nature of their aglycone moieties. We have identified a cytochrome P450 gene (CYP716A12) involved in saponin synthesis in Medicago truncatula using a combined genetic and biochemical approach. Genetic loss-of-function analysis and complementation studies showed that CYP716A12 is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 were unable to produce hemolytic saponins and only synthetized soyasaponins, and were thus named lacking hemolytic activity (lha). In vitro enzymatic activity assays indicate that CYP716A12 catalyzes the oxidation of β-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid. Transcriptome changes in the lha mutant showed a modulation in the main steps of triterpenic saponin biosynthetic pathway: squalene cyclization, β-amyrin oxidation, and glycosylation. The analysis of CYP716A12 expression in planta is reported together with the sapogenin content in different tissues and stages. This article provides evidence for CYP716A12 being a key gene in hemolytic saponin biosynthesis.
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