ArticleApplied and environmental microbiology2011
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species.Applied microbiology and biotechnology · 2026Article
- Evolving a plant-beneficial bacterium in soil vs. nutrient-rich liquid culture has contrasting effects on in-soil fitness.Applied and environmental microbiology · 2025Article
- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Rampant loss of social traits during domestication of a Bacillus subtilis natural isolate.Scientific reports · 2020Article
- Experimental Evolution ofGenetics · 2020Article
- Microbial life cycles link global modularity in regulation to mosaic evolution.Nature ecology & evolution · 2019Article
- Genome analysis of a Bacillus subtilis strain reveals genetic mutations determining biocontrol properties.World journal of microbiology & biotechnology · 2019Article
- Genetics of dispersal.Biological reviews of the Cambridge Philosophical Society · 2018Review
- Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain.Genome announcements · 2016Article
- Experimental evolution of enhanced growth by Bacillus subtilis at low atmospheric pressure: genomic changes revealed by whole-genome sequencing.Applied and environmental microbiology · 2015Article
- Optical Whole-Genome Restriction Mapping as a Tool for Rapidly Distinguishing and Identifying Bacterial Contaminants in Clinical Samples.Journal of clinical and diagnostic research : JCDR · 2015Article
- Genome dynamics during experimental evolution.Nature reviews. Genetics · 2013Review
- Complete Genome Sequence of Mycoplasma hyorhinis Strain SK76.Genome announcements · 2013Article
- Comparative Genomics of Bacillus species and its Relevance in Industrial Microbiology.Genomics insights · 2013Review
- BAC-pool sequencing and analysis of large segments of A12 and D12 homoeologous chromosomes in upland cotton.PloS one · 2013Article
- Effect of the Bacillus atrophaeus subsp. globigii Spo0F H101R mutation on strain fitness.Applied and environmental microbiology · 2012Article
- Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigW.Applied and environmental microbiology · 2012Article
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Corrections and comments
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about how genetic variation at the nucleotide level contributes to competitive fitness within species. During a 6,000-generation study of Bacillus subtilis evolved under relaxed selection for sporulation, a new strain, designated WN716, emerged with significantly different colony and cell morphologies; loss of sporulation, competence, acetoin production, and motility; multiple auxotrophies; and increased competitive fitness (H. Maughan and W. L. Nicholson, Appl. Environ. Microbiol. 77:4105-4118, 2011). The genome of WN716 was analyzed by OpGen optical mapping, whole-genome 454 pyrosequencing, and the CLC Genomics Workbench. No large chromosomal rearrangements were found; however, 34 single-nucleotide polymorphisms (SNPs) and +1 frameshifts were identified in WN716 that resulted in amino acid changes in coding sequences of annotated genes, and 11 SNPs were located in intergenic regions. Several classes of genes were affected, including biosynthetic pathways, sporulation, competence, and DNA repair. In several cases, attempts were made to link observed phenotypes of WN716 with the discovered mutations, with various degrees of success. For example, a +1 frameshift was identified at codon 13 of sigW, the product of which (SigW) controls a regulon of genes involved in resistance to bacteriocins and membrane-damaging antibiotics. Consistent with this finding, WN716 exhibited sensitivity to fosfomycin and to a bacteriocin produced by B. subtilis subsp. spizizenii and exhibited downregulation of SigW-dependent genes on a transcriptional microarray, consistent with WN716 carrying a knockout of sigW. The results suggest that propagation of B. subtilis for less than 2,000 generations in a nutrient-rich environment where sporulation is suppressed led to rapid initiation of genomic erosion.
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