ArticleBlood2011
Development of thymic NK cells from double negative 1 thymocyte precursors.
Article in Blood, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Radioresistant intrathymic stem cells: retrospective analysis and concept of the role in thymic oncogenesis and post-irradiation regeneration.Frontiers in immunology · 2026Review
- A human NK cell progenitor that originates in the thymus and generates KIRScience advances · 2025Article
- Unraveling NK cell heterogeneity through single-cell sequencing: insights from physiological and tumor contexts for clinical applications.Frontiers in immunology · 2025Review
- Feeder-cell-free system forFrontiers in immunology · 2025Article
- A timed epigenetic switch balances T and ILC lineage proportions in the thymus.Development (Cambridge, England) · 2024Article
- Type 1 immunity enables neonatal thymic ILC1 production.Science advances · 2024Article
- Thymic NK-Cells and Their Potential in Cancer Immunotherapy.ImmunoTargets and therapy · 2024Review
- Natural killer cells and their exosomes in viral infections and related therapeutic approaches: where are we?Cell communication and signaling : CCS · 2023Review
- Article
- Delta-like 4-Derived Notch Signals Differentially Regulate Thymic Generation of Skin-Homing CCR10Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Article
- The divergence between T cell and innate lymphoid cell fates controlled by E and Id proteins.Frontiers in immunology · 2022Review
- Natural Killer Cell Integrins and Their Functions in Tissue Residency.Frontiers in immunology · 2021Review
- Review
- Transcriptional Regulation of Mouse Tissue-Resident Natural Killer Cell Development.Frontiers in immunology · 2020Review
- Review
- Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors.The Journal of experimental medicine · 2019Article
- NFIL3 Expression Distinguishes Tissue-Resident NK Cells and Conventional NK-like Cells in the Mouse Submandibular Glands.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Phenotype and functions of conventional and non-conventional NK cells.Current opinion in immunology · 2016Review
- Type 1 Innate Lymphoid Cell Biology: Lessons Learnt from Natural Killer Cells.Frontiers in immunology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The differentiation of natural killer (NK) cells and a subpopulation of NK cells which requires an intact thymus, that is, thymic NK cells, is poorly understood. Previous in vitro studies indicate that double negative (CD4⁻CD8⁻, DN) thymocytes can develop into cells with NK cell markers, but these cells have not been well characterized. Herein, we generated and characterized NK cells differentiating from thymic DN precursors. Sorted DN1 (CD44⁺CD25⁻) CD122⁻NK1.1⁻ thymocytes from Rag1(⁻/⁻) mice were adoptively transferred into Rag1(⁻/⁻)Ly5.1 congenic mice. After intrathymic injection, donor-derived cells phenotypically resembling thymic NK cells were found. To further study their differentiation, we seeded sorted DN1 CD122⁻)NK1.1⁻ thymocytes on irradiated OP9 bone marrow stromal cells with IL-15, IL-7, Flt3L, and stem cell factor. NK1.1⁺ cells emerged after 7 days. In vitro differentiated NK cells acquired markers associated with immature bone marrow-derived NK cells, but also expressed CD127, which is typically found on thymic NK cells. Furthermore, we found that in vitro cells generated from thymic precursors secreted cytokines when stimulated and degranulated on target exposure. Together, these data indicate that functional thymic NK cells can develop from a DN1 progenitor cell population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.