ArticleAnalytical biochemistry2011
Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Bioenergetic profiles and respiratory control in mitochondrial physiology: Precision analysis of oxidative phosphorylation.Experimental physiology · 2026Article
- Rethinking mitochondrial isolation for the next generation of mitochondrial therapies.Frontiers in physiology · 2026Review
- Review
- Article
- Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes.Bio-protocol · 2019Article
- A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics.Communications biology · 2019Article
- Mitochondrial GSH replenishment as a potential therapeutic approach for Niemann Pick type C disease.Redox biology · 2017Article
- The renal mitochondrial dysfunction in patients with vascular calcification is prevented by sodium thiosulfate.International urology and nephrology · 2016Article
- Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples.Scientific reports · 2016Article
- Article
- Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements.Journal of visualized experiments : JoVE · 2015Article
- High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria.Biomolecules · 2015Article
- Preparation and respirometric assessment of mitochondria isolated from skeletal muscle tissue obtained by percutaneous needle biopsy.Journal of visualized experiments : JoVE · 2015Article
- Rapid isolation and purification of mitochondria for transplantation by tissue dissociation and differential filtration.Journal of visualized experiments : JoVE · 2014Article
- Article
- Review on recent advances in the analysis of isolated organelles.Analytica chimica acta · 2012Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Isolation of functional and intact mitochondria from solid tissue is crucial for studies that focus on the elucidation of normal mitochondrial physiology and/or mitochondrial dysfunction in conditions such as aging, diabetes, and cancer. There is growing recognition of the importance of mitochondria both as targets for drug development and as off-target mediators of drug side effects. Unfortunately, mitochondrial isolation from tissue is generally carried out using homogenizer-based methods that require extensive operator experience to obtain reproducible high-quality preparations. These methods limit dissemination, impede scale-up, and contribute to difficulties in reproducing experimental results over time and across laboratories. Here we describe semiautomated methods to disrupt tissue using kidney and muscle mitochondria preparations as exemplars. These methods use the Barocycler, the PCT Shredder, or both. The PCT Shredder is a mechanical grinder that quickly breaks up tissue without significant risk of overhomogenization. Mitochondria isolated using the PCT Shredder are shown to be comparable to controls. The Barocycler generates controlled pressure pulses that can be adjusted to lyse cells and release organelles. The mitochondria subjected to pressure cycling-mediated tissue disruption are shown to retain functionality, enabling combinations of the PCT Shredder and the Barocycler to be used to purify mitochondrial preparations.
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Registered trials
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