ArticleBiomaterials2011
Changes of chondrocyte expression profiles in human MSC aggregates in the presence of PEG microspheres and TGF-β3.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Mesenchymal Stromal Cell Chondrogenic Differentiation Induced by Continuous Stiffness Gradient in Photocrosslinkable Hydrogels.Journal of biomedical materials research. Part A · 2025Article
- Injectable MSC Spheroid and Microgel Granular Composites for Engineering Tissue.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Engineered biomaterials to guide spheroid formation, function, and fabrication into 3D tissue constructs.Acta biomaterialia · 2023Review
- Treatment and application of stem cells from different sources for cartilage injury: a literature review.Annals of translational medicine · 2022Review
- Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells.BioMed research international · 2021Article
- Dual functional construct containing kartogenin releasing microtissues and curcumin for cartilage regeneration.Stem cell research & therapy · 2020Article
- Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids.Frontiers in bioengineering and biotechnology · 2020Article
- miR-483 targets SMAD4 to suppress chondrogenic differentiation of human mesenchymal stem cells.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Angiogenic Potential of Human Bone Marrow-Derived Mesenchymal Stem Cells in Chondrocyte Brick-Enriched Constructs Promoted Stable Regeneration of Craniofacial Cartilage.Stem cells translational medicine · 2017Article
- Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells.International journal of nanomedicine · 2017Article
- A modular, plasmin-sensitive, clickable poly(ethylene glycol)-heparin-laminin microsphere system for establishing growth factor gradients in nerve guidance conduits.Biomaterials · 2015Article
- Gene expression modulation in TGF-β3-mediated rabbit bone marrow stem cells using electrospun scaffolds of various stiffness.Journal of cellular and molecular medicine · 2015Article
- Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint.Scientific reports · 2015Article
- Alternative splicing of type II procollagen: IIB or not IIB?Connective tissue research · 2014Review
- Controlled local presentation of matrix proteins in microparticle-laden cell aggregates.Biotechnology and bioengineering · 2014Article
- Chondroitin sulfate microparticles modulate transforming growth factor-β1-induced chondrogenesis of human mesenchymal stem cell spheroids.Cells, tissues, organs · 2014Article
- Controlled release strategies for bone, cartilage, and osteochondral engineering--Part I: recapitulation of native tissue healing and variables for the design of delivery systems.Tissue engineering. Part B, Reviews · 2013Review
- High-density cell systems incorporating polymer microspheres as microenvironmental regulators in engineered cartilage tissues.Tissue engineering. Part B, Reviews · 2013Review
- Quantification of type II procollagen splice forms using alternative transcript-qPCR (AT-qPCR).Matrix biology : journal of the International Society for Matrix BiologyArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Biomaterial microparticles are commonly utilized as growth factor delivery vehicles to induce chondrogenic differentiation of mesenchymal stem/stromal cells (MSCs). To address whether the presence of microparticles could themselves affect differentiation of MSCs, a 3D co-aggregate system was developed containing an equal volume of human primary bone marrow-derived MSCs and non-degradable RGD-conjugated poly(ethylene glycol) microspheres (PEG-μs). Following TGF-β3 induction, differences in cell phenotype, gene expression and protein localization patterns were found when compared to MSC aggregate cultures devoid of PEG-μs. An outer fibrous layer always found in differentiated MSC aggregate cultures was not formed in the presence of PEG-μs. Type II collagen protein was synthesized by cells in both culture systems, although increased levels of the long (embryonic) procollagen isoforms were found in MSC/PEG-μs aggregates. Ubiquitous deposition of type I and type X collagen proteins was found in MSC/PEG-μs cultures while the expression patterns of these collagens was restricted to specific areas in MSC aggregates. These findings show that MSCs respond differently to TGF-β3 when in a PEG-μs environment due to effects of cell dilution, altered growth factor diffusion and/or cellular interactions with the microspheres. Although not all of the expression patterns pointed toward improved chondrogenic differentiation in the MSC/PEG-μs cultures, the surprisingly large impact of the microparticles themselves should be considered when designing drug delivery/scaffold strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.