ArticleJournal of microbiological methods2011
Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption.
Article in Journal of microbiological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- NaMeco - Nanopore full-length 16S rRNA gene reads clustering and annotation.BMC genomics · 2025Article
- Interplay between WNT/PI3K-mTOR axis and the microbiota in APC-driven colorectal carcinogenesis: data from a pilot study and possible implications for CRC prevention.Journal of translational medicine · 2024Article
- The gut ileal mucosal virome is disturbed in patients with Crohn's disease and exacerbates intestinal inflammation in mice.Nature communications · 2024Article
- Optimized bacterial DNA isolation method for microbiome analysis of human tissues.MicrobiologyOpen · 2021Article
- Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity.Scientific reports · 2017Article
- Sampling Strategies for Three-Dimensional Spatial Community Structures in IBD Microbiota Research.Frontiers in cellular and infection microbiology · 2017Review
- A protocol for the subcellular fractionation of Saccharomyces cerevisiae using nitrogen cavitation and density gradient centrifugation.Yeast (Chichester, England) · 2014Article
- DNA extract characterization process for microbial detection methods development and validation.BMC research notes · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.