ArticlePlant molecular biology2011
RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 93 citations in OpenAlex.
- Genome-Wide Genetic Architecture for Common Scab (International journal of molecular sciences · 2025Article
- Expansion and functional divergence of terpene synthase genes in angiosperms: a driving force of terpene diversity.Horticulture research · 2025Article
- Transcriptome-wide investigation and functional characterization reveal a terpene synthase involved in γ-terpinene biosynthesis in Monarda citriodora.Functional & integrative genomics · 2024Article
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- Cloning, Expression and Functional Characterization of a Novel α-Humulene Synthase, Responsible for the Formation of Sesquiterpene in Agarwood Originating fromCurrent issues in molecular biology · 2023Article
- Germacrene B - a central intermediate in sesquiterpene biosynthesis.Beilstein journal of organic chemistry · 2023Review
- Genetic and physiological requirements for high-level sesquiterpene-production in tomato glandular trichomes.Frontiers in plant science · 2023Article
- Combined transcriptome and metabolome analysis of Nerium indicum L. elaborates the key pathways that are activated in response to witches' broom disease.BMC plant biology · 2022Article
- Regulation of Tomato Specialised Metabolism after Establishment of Symbiosis with the Endophytic FungusMicroorganisms · 2022Article
- A high-efficiency trichome collection system by laser capture microdissection.Frontiers in plant science · 2022Article
- Taxonomic Insights and Its Type Cyclization Correlation of Volatile Sesquiterpenes inMolecules (Basel, Switzerland) · 2021Review
- Article
- Glandular Trichome-Derived Mono- and Sesquiterpenes of Tomato Have Contrasting Roles in the Interaction with the Potato Aphid Macrosiphum euphorbiae.Journal of chemical ecology · 2021Article
- Epidermis-Specific Metabolic Engineering of Sesquiterpene Formation in Tomato Affects the Performance of Potato AphidFrontiers in plant science · 2021Article
- Plant Volatile Organic Compounds Evolution: Transcriptional Regulation, Epigenetics and Polyploidy.International journal of molecular sciences · 2020Review
- The complete functional characterisation of the terpene synthase family in tomato.The New phytologist · 2020Article
- Combinatorial Evolution of a Terpene Synthase Gene Cluster Explains Terpene Variations inPlant physiology · 2020Article
- An integrated strategy to identify genes responsible for sesquiterpene biosynthesis in turmeric.Plant molecular biology · 2019Article
- Comparative transcriptome analyses on terpenoids metabolism in field- and mountain-cultivated ginseng roots.BMC plant biology · 2019Article
- From Central to Specialized Metabolism: An Overview of Some Secondary Compounds Derived From the Primary Metabolism for Their Role in Conferring Nutritional and Organoleptic Characteristics to Fruit.Frontiers in plant science · 2019Review
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S. lycopersicum genome resulted in the discovery of two additional sesquiterpene synthases expressed in trichomes. The sesquiterpene synthases from S. lycopersicum and S. habrochaites have high levels of protein identity. Several of them appeared to encode for non-functional proteins. Functional recombinant proteins produced germacrenes, β-caryophyllene/α-humulene, viridiflorene and valencene from (E,E)-farnesyl diphosphate. However, the activities of these enzymes do not completely explain the differences in sesquiterpene production between the two tomato plants. RT-qPCR confirmed high levels of expression of most of the S. lycopersicum sesquiterpene synthases in stem trichomes. In addition, one sesquiterpene synthase was induced by jasmonic acid, while another appeared to be slightly repressed by the treatment. Our data provide a foundation to study the evolution of terpene synthases in cultivated and wild tomato.
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