ArticlePloS one2011
Identification of host genes involved in geminivirus infection using a reverse genetics approach.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 73 citations in OpenAlex.
- Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection.The New phytologist · 2026Article
- A SUMO interacting motif in the replication initiator protein of tomato yellow leaf curl virus is required for viral replication.Journal of virology · 2025Article
- Review
- A reporter tomato line to track replication of a geminivirus in real time and with cellular resolution.Plant biotechnology journal · 2025Article
- Review
- Transcriptional Analysis of the Differences between ToLCNDV-India and ToLCNDV-ES Leading to Contrary Symptom Development in Cucumber.International journal of molecular sciences · 2023Article
- In-silico evolutionary analysis of plant-OBERON proteins during compatible MYMV infection in respect of improving host resistance.Journal of plant research · 2022Article
- Members of the ribosomal protein S6 (RPS6) family act as pro-viral factor for tomato spotted wilt orthotospovirus infectivity in Nicotiana benthamiana.Molecular plant pathology · 2022Article
- The interplay of plant hormonal pathways and geminiviral proteins: partners in disease development.Virus genes · 2022Review
- Article
- Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton (Gossypium hirsutum).Plant biotechnology journal · 2020Article
- Next-Generation Sequencing and the CRISPR-Cas Nexus: A Molecular Plant Virology Perspective.Frontiers in microbiology · 2020Review
- Control of Plant Viruses by CRISPR/Cas System-Mediated Adaptive Immunity.Frontiers in microbiology · 2020Review
- Transcriptional reprogramming caused by the geminivirus Tomato yellow leaf curl virus in local or systemic infections in Nicotiana benthamiana.BMC genomics · 2019Article
- Plant defense against virus diseases; growth hormones in highlights.Plant signaling & behavior · 2019Review
- Modifying the Replication of Geminiviral Vectors Reduces Cell Death and Enhances Expression of Biopharmaceutical Proteins inFrontiers in plant science · 2018Article
- Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots.Plant methods · 2018Article
- Article
- Review
- No evidence of seed transmissibility of Tomato yellow leaf curl virus in Nicotiana benthamiana.Journal of Zhejiang University. Science. B · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geminiviruses, like all viruses, rely on the host cell machinery to establish a successful infection, but the identity and function of these required host proteins remain largely unknown. Tomato yellow leaf curl Sardinia virus (TYLCSV), a monopartite geminivirus, is one of the causal agents of the devastating Tomato yellow leaf curl disease (TYLCD). The transgenic 2IRGFP N. benthamiana plants, used in combination with Virus Induced Gene Silencing (VIGS), entail an important potential as a tool in reverse genetics studies to identify host factors involved in TYLCSV infection. Using these transgenic plants, we have made an accurate description of the evolution of TYLCSV replication in the host in both space and time. Moreover, we have determined that TYLCSV and Tobacco rattle virus (TRV) do not dramatically influence each other when co-infected in N. benthamiana, what makes the use of TRV-induced gene silencing in combination with TYLCSV for reverse genetic studies feasible. Finally, we have tested the effect of silencing candidate host genes on TYLCSV infection, identifying eighteen genes potentially involved in this process, fifteen of which had never been implicated in geminiviral infections before. Seven of the analyzed genes have a potential anti-viral effect, whereas the expression of the other eleven is required for a full infection. Interestingly, almost half of the genes altering TYLCSV infection play a role in postranslational modifications. Therefore, our results provide new insights into the molecular mechanisms underlying geminivirus infections, and at the same time reveal the 2IRGFP/VIGS system as a powerful tool for functional reverse genetics studies.
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