ArticleMicrobiology (Reading, England)2011
Transcriptome analysis reveals that ClpXP proteolysis controls key virulence properties of Streptococcus mutans.
Article in Microbiology (Reading, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- MecA: A Multifunctional ClpP-Dependent and Independent Regulator in Gram-Positive Bacteria.Molecular microbiology · 2025Review
- MecA inmSphere · 2024Article
- Engineering a nanoantibiotic system displaying dual mechanism of action.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome ofMicrobiology spectrum · 2024Article
- Diverse nature of ClpX degradation motifs inMicrobiology spectrum · 2024Article
- The quorum sensing peptide BlpC regulates the transcription of genes outside its associated gene cluster and impacts the growth ofFrontiers in microbiology · 2023Article
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.Journal of bacteriology · 2022Article
- Characterization of FtsH Essentiality inFrontiers in genetics · 2021Article
- Carbohydrate and PepO control bimodality in competence development by Streptococcus mutans.Molecular microbiology · 2019Article
- Deficiency of MecA inFrontiers in microbiology · 2018Article
- Inactivation of the spxA1 or spxA2 gene of Streptococcus mutans decreases virulence in the rat caries model.Molecular oral microbiology · 2017Article
- Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't.Molecular oral microbiology · 2017Review
- Mechanistic, genomic and proteomic study on the effects of BisGMA-derived biodegradation product on cariogenic bacteria.Dental materials : official publication of the Academy of Dental Materials · 2017Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Structure and Functional Properties of the Active Form of the Proteolytic Complex, ClpP1P2, from Mycobacterium tuberculosis.The Journal of biological chemistry · 2016Article
- An Essential Role for (p)ppGpp in the Integration of Stress Tolerance, Peptide Signaling, and Competence Development in Streptococcus mutans.Frontiers in microbiology · 2016Article
- Meta-omics uncover temporal regulation of pathways across oral microbiome genera during in vitro sugar metabolism.The ISME journal · 2015Article
- A unique open reading frame within the comX gene of Streptococcus mutans regulates genetic competence and oxidative stress tolerance.Molecular microbiology · 2015Article
- Role of the Streptococcus mutans CRISPR-Cas systems in immunity and cell physiology.Journal of bacteriology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ClpXP proteolytic complex is critical for maintaining cellular homeostasis, as well as expression of virulence properties. However, with the exception of the Spx global regulator, the molecular mechanisms by which the ClpXP complex exerts its influence in Streptococcus mutans are not well understood. Here, microarray analysis was used to provide novel insights into the scope of ClpXP proteolysis in S. mutans. In a ΔclpP strain, 288 genes showed significant changes in relative transcript amounts (P≤0.001, twofold cut-off) as compared with the parent. Similarly, 242 genes were differentially expressed by a ΔclpX strain, 113 (47 %) of which also appeared in the ΔclpP microarrays. Several genes associated with cell growth were downregulated in both mutants, consistent with the slow-growth phenotype of the Δclp strains. Among the upregulated genes were those encoding enzymes required for the biosynthesis of intracellular polysaccharides (glg genes) and malolactic fermentation (mle genes). Enhanced expression of glg and mle genes in ΔclpP and ΔclpX strains correlated with increased storage of intracellular polysaccharide and enhanced malolactic fermentation activity, respectively. Expression of several genes known or predicted to be involved in competence and mutacin production was downregulated in the Δclp strains. Follow-up transformation efficiency and deferred antagonism assays validated the microarray data by showing that competence and mutacin production were dramatically impaired in the Δclp strains. Collectively, our results reveal the broad scope of ClpXP regulation in S. mutans homeostasis and identify several virulence-related traits that are influenced by ClpXP proteolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.