Structural insight into the low affinity between Thermotoga maritima CheA and CheB compared to their Escherichia coli/Salmonella typhimurium counterparts.

Sangyoun Park, Brian R Crane

Open access · greenAbstract readComparative Study

In one paragraph

Article in International journal of biological macromolecules, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 90% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Sangyoun ParkSchool of Systems Biomedical Science, College of Natural Sciences, Soongsil University, Seoul, Republic of Korea. psy@ssu.ac.kr

Brian R Crane

Cornell University · USSoongsil University · KR

Funding

The Structure and Function of the Bacterial ChemosomeR01GM066775 · NIGMS · CORNELL UNIVERSITY ITHACA · PI CRANE, BRIAN R · 2003 to 2016

$4.3M

NIGMS NIH HHS GM066775NIGMS NIH HHS R01 GM066775

6 · The paper itself

Abstract

CheA-mediated CheB phosphorylation and the subsequent CheB-mediated demethylation of the chemoreceptors are important steps required for the bacterial chemotactic adaptation response. Although Escherichia coli CheB has been reported to interact with CheA competitively against CheY, we have observed that Thermotoga maritima CheB has no detectable CheA-binding. By determining the CheY-like domain crystal structure of T. maritima CheB, and comparing against the T. maritima CheY and Salmonella typhimurium CheB structures, we propose that the two consecutive glutamates in the β4/α4 loop of T. maritima CheB that is absent in T. maritima CheY and in E. coli/S. typhimurium CheB may be one factor contributing to the low CheA affinity.

Indexed as

Amino Acid SequenceBacterial ProteinsCrystallography, X-RayEscherichia coliEscherichia coli ProteinsHistidine KinaseMembrane ProteinsMethyl-Accepting Chemotaxis ProteinsModels, MolecularMolecular Sequence DataProtein Structure, SecondaryProtein Structure, TertiarySalmonella typhimuriumSequence AlignmentStructure-Activity RelationshipThermotoga maritimaBacterial ProteinscheA protein, E coliCheB protein, BacteriacheY protein, E coliEscherichia coli ProteinsHistidine KinaseMembrane ProteinsMethyl-Accepting Chemotaxis Proteins

Identifiers

PMID21816169

DOI10.1016/j.ijbiomac.2011.07.012

PMCPMC3204391

OpenAlexW2027099377

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.