ArticleBiochemistry2011
Permeation of styryl dyes through nanometer-scale pores in membranes.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Synthetic cells in biomedical applications.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Pan-Drug ResistantAntibiotics (Basel, Switzerland) · 2020Article
- How Melittin Inserts into Cell Membrane: Conformational Changes, Inter-Peptide Cooperation, and Disturbance on the Membrane.Molecules (Basel, Switzerland) · 2019Review
- Membrane Active Peptides and Their Biophysical Characterization.Biomolecules · 2018Review
- Engineered transmembrane pores.Current opinion in chemical biology · 2016Review
- Review
- A new role for myosin II in vesicle fission.PloS one · 2014Article
- Process of inducing pores in membranes by melittin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Properties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Article
- Obstructing toxin pathways by targeted pore blockage.Chemical reviews · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Styryl dyes are widely used to study synaptic vesicle (SV) recycling in neurons; vesicles are loaded with dye during endocytosis, and dye is subsequently released via exocytosis. During putative kiss-and-run exocytosis, efflux of dye from individual SVs has been proposed to occur via two sequential steps: dissociation from the membrane followed by permeation through a small fusion pore. To improve our understanding of the kinetics of efflux of dye from vesicles during kiss-and-run events, we examined the rates of efflux of different dyes through nanometer-scale pores formed in membranes by the toxins melittin and α-hemolysin; these pores approximate the size of fusion pores measured in neuroendocrine cells. We found that the axial diameter of each dye was a crucial determinant for permeation. Moreover, the two dyes with the largest cross-sectional areas were completely unable to pass through pores formed by a mutant α-hemolysin that has a slightly smaller pore than the wild-type toxin. The overall time constant for efflux (seconds) of each dye was orders of magnitude slower than the time constant for dissociation from membranes (milliseconds). Thus, the permeation step is rate-limiting, and this observation was further supported by atomistic molecular dynamics simulations. Together, the data reported here help provide a framework for interpreting dye destaining rates from secretory vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.