ArticleMethods in molecular biology (Clifton, N.J.)2011
Genome engineering using targeted oligonucleotide libraries and functional selection.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection ofACS synthetic biology · 2026Article
- Adaptive laboratory evolution ofFrontiers in microbiology · 2026Article
- Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.The EMBO journal · 2025Article
- Glycogen phase separation drives macromolecular rearrangement and asymmetric division inbioRxiv : the preprint server for biology · 2025Article
- Strain-specific galactose utilization by commensal E. coli mitigates Salmonella establishment in the gut.PLoS pathogens · 2025Article
- Commensal E. coli limits Salmonella gut invasion during inflammation by producing toxin-bound siderophores in a tonB-dependent manner.PLoS biology · 2024Article
- Assessing microbiome population dynamics using wild-type isogenic standardized hybrid (WISH)-tags.Nature microbiology · 2024Article
- Non-pathogenic Escherichia coli acquires virulence by mutating a growth-essential LPS transporter.PLoS pathogens · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The λ phage Red proteins greatly enhance homologous recombination in Escherichia coli. Red-mediated recombination or "recombineering" can be used to construct targeted gene deletions as well as to introduce point mutations into the genome. Here, we describe our method for scanning mutagenesis using recombineered oligonucleotide libraries. This approach entails randomization of specific codons within a target gene, followed by functional selection to isolate mutants. Oligonucleotide library mutagenesis has generated hundreds of novel antibiotic resistance mutations in genes encoding ribosomal proteins, and should be applicable to other systems for which functional selections exist.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.