ArticleThe journal of physical chemistry letters2010
Real-Time Infrared Overtone Laser Control of Temperature in Picoliter H(2)O Samples: "Nanobathtubs" for Single Molecule Microscopy.
Article in The journal of physical chemistry letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Development of label-free light-controlled gene expression technologies using mid-IR and terahertz light.Frontiers in bioengineering and biotechnology · 2024Review
- Light-driven nucleation, growth, and patterning of biorelevant crystals using resonant near-infrared laser heating.Nature communications · 2023Article
- Temperature-Jump 2D IR Spectroscopy with Intensity-Modulated CW Optical Heating.The journal of physical chemistry. B · 2020Article
- A Temperature-Jump Optical Trap for Single-Molecule Manipulation.Biophysical journal · 2015Article
- Pulsed IR heating studies of single-molecule DNA duplex dissociation kinetics and thermodynamics.Biophysical journal · 2014Article
- Single-molecule kinetics reveal cation-promoted DNA duplex formation through ordering of single-stranded helices.Biophysical journal · 2013Article
- Temperature sculpting in yoctoliter volumes.Journal of the American Chemical Society · 2013Article
- Established and novel methods of interrogating two-dimensional cell migration.Integrative biology : quantitative biosciences from nano to macro · 2012Review
- Thermodynamic origins of monovalent facilitated RNA folding.Biochemistry · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
An approach for high spatiotemporal control of aqueous sample temperatures in confocal microscopy is reported. This technique exploits near-IR diode-laser illumination to locally heat picoliter volumes of water via first-overtone excitation in the OH-stretch manifold. A thin water cell after the objective resonantly removes any residual IR light from the detection system, allowing for continuous observation of single-molecule fluorescence throughout the heating event. This technique is tested quantitatively by reproducing single-molecule RNA folding results obtained from "bulk" stage heating measurements. Calibration of sample temperatures is obtained from time-correlated single-photon counting studies of Rhodamine B fluorescence decay. We obtain an upper limit to the heating response time (τ(heat) < 20 ms) consistent with even faster estimates (τ(heat) ≈ 0.25 ms) based on laser spot size, H(2)O heat capacit,y and absorption cross section. This combination of fast, noncontact heating of picoliter volumes provides new opportunities for real-time thermodynamic/kinetic studies at the single-molecule level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.