ArticleInternational journal of biological sciences2011
Long-term cultured human neural stem cells undergo spontaneous transformation to tumor-initiating cells.
Article in International journal of biological sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment.International journal of molecular sciences · 2023Review
- Role of hypoxic exosomes and the mechanisms of exosome release in the CNS under hypoxic conditions.Frontiers in neurology · 2023Review
- Effect of Placenta-Derived Mesenchymal Stromal Cells Conditioned Media on an LPS-Induced Mouse Model of Preeclampsia.International journal of molecular sciences · 2022Article
- Combination of tucatinib and neural stem cells secreting anti-HER2 antibody prolongs survival of mice with metastatic brain cancer.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Targeting Brain-Adaptive Cancer Stem Cells Prohibits Brain Metastatic Colonization of Triple-Negative Breast Cancer.Cancer research · 2018Article
- Nicorandil potentiates sodium butyrate induced preconditioning of neurons and enhances their survival upon subsequent treatment with HTranslational neurodegeneration · 2017Article
- Mesenchymal stem cells with irreversibly arrested proliferation stimulate decidua development in rats.Experimental and therapeutic medicine · 2016Article
- A practical way to prepare primer human chondrocyte culture.Journal of orthopaedics · 2016Article
- Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy.Stem cells international · 2016Article
- Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies?Genes & nutrition · 2014Article
- Identification of novel biomarkers in neuroblastoma associated with the risk for bone marrow metastasis: a pilot study.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2013Article
- Stem cell transplantation for neuroprotection in stroke.Brain sciences · 2013Article
- Genetic instability in neural stem cells: an inconvenient truth?The Journal of clinical investigation · 2012Article
- Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells.The Journal of clinical investigation · 2012Article
- Spontaneous transformation of stem cells in vitro and the issue of cross-contamination.International journal of biological sciences · 2012Article
- Safety of mesenchymal stem cells for clinical application.Stem cells international · 2012Article
- Neurotransplantation: lux et veritas, fiction or reality?Journal of neurosurgical sciences · 2011Review
- Human pluripotent stem cell therapy for Huntington's disease: technical, immunological, and safety challenges human pluripotent stem cell therapy for Huntington's disease: technical, immunological, and safety challenges.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2011Article
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10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report, we describe the spontaneous malignant transformation of long-term cultured human fetal striatum neural stem cells (hsNSCs, passage 17). After subcutaneous transplantation of long-term cultured hsNSCs into immunodeficient nude mice, 2 out of 15 mice formed xenografts which expressed neuroendocrine tumor markers CgA and NSE. T1 cells, a cell line that we derived from one of the two subcutaneous xenografts, have undergone continuous expansion in vitro. These T1 cells showed stem cell-like features and expressed neural stem cell markers nestin and CD133. The T1 cells were involved in abnormal karyotype, genomic instability and fast proliferation. Importantly, after long-term in vitro culture, the T1 cells did not result in subcutaneous xenografts, but induced intracranial tumor formation, indicating that they adjusted themselves to the intracranial microenvironment. We further found that the T1 cells exhibited an overexpressed level of EGFR, and the CD133 positive T1 cells showed a truncation mutation in the exons 2-7 of the EGFR (EGFRvIII) gene. These results suggest that continuous expansion of neural stem cells in culture may lead to malignant spontaneous transformation. This phenomenon may be functionally related to EGFR by EGFRvIII gene mutation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.