ArticleProceedings of the National Academy of Sciences of the United States of America1990
Hamster cell line suitable for transfection assay of transforming genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- KRAS and NRAS mutational gene profile of metastatic colorectal cancer patients in Jordan.PloS one · 2019Article
- Activating K-Ras mutations outwith 'hotspot' codons in sporadic colorectal tumours - implications for personalised cancer medicine.British journal of cancer · 2010Article
- A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA-induced IL-12 production and Th cell differentiation.The Journal of clinical investigation · 2004Article
- The virulence plasmid of Yersinia, an antihost genome.Microbiology and molecular biology reviews : MMBR · 1998Review
- The Yersinia deadly kiss.Journal of bacteriology · 1998Review
- Mutations in ras genes in cells cultured from mouse skin tumors induced by ultraviolet irradiation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H(+)-ATPase.In vitro cellular & developmental biology. Animal · 1993Article
- The murine cot proto-oncogene: genome structure and tissue-specific expression.Japanese journal of cancer research : Gann · 1993Article
- Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours.British journal of cancer · 1993Article
- X-ray crystal structures of transforming p21 ras mutants suggest a transition-state stabilization mechanism for GTP hydrolysis.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Structure and transforming potential of the human cot oncogene encoding a putative protein kinase.Molecular and cellular biology · 1991Article
- In vitro progression-associated c-H-ras activation in neoplastic hepatocyte lines established from SV40-T antigen gene-harboring transgenic mice.Japanese journal of cancer research : Gann · 1991Article
- A novel point mutation at codon 146 of the K-ras gene in a human colorectal cancer identified by the polymerase chain reaction.Virus genes · 1991Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established a subclone, SHOK, from the GHE-L cell line, an immortal line derived from a primary culture of Syrian hamster embryo cells, as a recipient cell line useful for the detection of oncogenes by transfection. SHOK cells were almost as susceptible as NIH 3T3 cells to focus formation by many oncogenes, including v-raf, v-Ha-ras, v-Ki-ras, or activated c-Ha-ras. The susceptibility of SHOK to focus formation was higher than that of NIH 3T3 for v-mos but was lower for v-fps, v-fgr, v-src, v-sis, and v-abl. When DNAs extracted from 27 human and murine tumors were tested for focus formation, 5 DNAs were positive in NIH 3T3 cells, whereas 9 were positive in SHOK cells at the primary transfection. Using SHOK cells as recipients of tumor cellular DNA, we isolated another oncogene and a c-Ki-ras2 gene mutated at codon 146 that were difficult to detect in NIH 3T3 cells. SHOK cells have a low rate of spontaneous transformation, produce easily distinguishable foci, and maintain a stable karyotype in transformed cells. In addition to being useful for the screening of human tumor DNAs, SHOK cells will be useful for the isolation of oncogenes from murine tumors because of their hamster origin.
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