ArticleProceedings of the National Academy of Sciences of the United States of America1990
Possible role of the Golgi apparatus in the assembly of very low density lipoprotein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Diagnostic and therapeutic potential of serum fatty acids in hyperlipidemia: evidence from three cohorts of patients with coronary atherosclerotic heart disease.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Fenofibrate Treatment Inhibits Very-Low-Density Lipoprotein Transport Vesicle Formation by Reducing Sar1b Protein Expression.International journal of molecular sciences · 2025Article
- Establishing the Role of Liver Fatty Acid-Binding Protein in Post-Golgi Very-Low-Density Lipoprotein Trafficking Using a Novel Fluorescence-Based Assay.International journal of molecular sciences · 2025Article
- Viral journeys on the intracellular highways.Cellular and molecular life sciences : CMLS · 2018Review
- Lipid droplets and liver disease: from basic biology to clinical implications.Nature reviews. Gastroenterology & hepatology · 2017Review
- CideB protein is required for the biogenesis of very low density lipoprotein (VLDL) transport vesicle.The Journal of biological chemistry · 2013Article
- Opposing roles of cell death-inducing DFF45-like effector B and perilipin 2 in controlling hepatic VLDL lipidation.Journal of lipid research · 2012Article
- Hepatic ABCA1 and VLDL triglyceride production.Biochimica et biophysica acta · 2012Review
- Intracellular trafficking and secretion of VLDL.Arteriosclerosis, thrombosis, and vascular biology · 2012Review
- Molecular determinants and dynamics of hepatitis C virus secretion.PLoS pathogens · 2012Article
- The identification of the SNARE complex required for the fusion of VLDL-transport vesicle with hepatic cis-Golgi.The Biochemical journal · 2010Article
- The many intersecting pathways underlying apolipoprotein B secretion and degradation.Trends in endocrinology and metabolism: TEM · 2008Review
- A role for palmitoylation in the quality control, assembly and secretion of apolipoprotein B.The Biochemical journal · 2004Article
- The assembly of hepatic very low density lipoproteins: evidence of a role for the Golgi apparatus.Lipids · 2002Article
- Identification of two regions in apolipoprotein B100 that are exposed on the cytosolic side of the endoplasmic reticulum membrane.The Journal of cell biology · 1998Article
- Triglycerides and disease.Postgraduate medical journal · 1993Review
- N-3 fatty acids stimulate intracellular degradation of apoprotein B in rat hepatocytes.The Journal of clinical investigation · 1993Article
- Function and regulation of expression of pulmonary surfactant-associated proteins.The Biochemical journal · 1991Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Transit of newly synthesized triacyl[3H]-glycerol through organelles of the secretory system leading to assembly into nascent very low density lipoproteins (VLDLs) or to cytoplasmic storage was investigated in chick hepatocytes. Cells in monolayer culture were pulse-labeled with [2-3H]glycerol, and after different periods of chase with unlabeled glycerol, the movement of triacyl[3H]glycerol through the endoplasmic reticulum (ER) and Golgi and the incorporation into nascent VLDL and cytoplasmic triacylglycerol-rich vesicles (TGRVs) were determined. Initially, newly synthesized triacyl[3H]glycerol is tightly associated with the ER. Movement from the ER of triacyl[3H]glycerol destined for cytoplasmic storage (as TGRVs) is extremely rapid and virtually complete within 8 min of chase. After 8 min of chase, triacyl[3H]glycerol lost from organelles of the secretory system was accounted for entirely as triacyl[3H]glycerol secreted as VLDL. Comparison of the rates of movement of triacyl[3H]glycerol, apolipoprotein B, apolipoprotein II, and apolipoprotein A-I through the ER and Golgi and of their secretion in nascent VLDL suggests that assembly of triacyglycerol with apolipoproteins occurs in the Golgi. Experiments with permeabilized hepatocytes supplemented with cytosol show that newly synthesized triacyl[3H]glycerol and [3H]phospholipid moves from the ER through the full-density range of Golgi elements and is dependent upon supplementary ATP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.