Evidence map›Paper›PMID 21813723›Full record

ArticleJournal of clinical microbiology2011

Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation.

M Kramski, A J Gaeguta, G F Lichtfuss, R Rajasuriar, S M Crowe, M A French, S R Lewin, R J Center, D F J Purcell

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

M KramskiDepartment of Microbiology and Immunology, The University of Melbourne, Parkville 3010, Victoria, Australia.
A J Gaeguta
G F Lichtfuss
R Rajasuriar
S M Crowe
M A French
S R Lewin
R J Center
D F J Purcell
Burnet Institute · AUThe University of Melbourne · AUThe University of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Indexed as

Bacterial TranslocationGenes, rRNABacteremiaBacteriological TechniquesDNA, BacterialHIV InfectionsHumansLipopolysaccharidesPlasmaReal-Time Polymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificityStatistics as TopicDNA, BacterialLipopolysaccharidesRNA, Ribosomal, 16S

Identifiers

PMID21813723
PMCPMC3187295
OpenAlexW2113347992

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.