ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Single-pixel optical fluctuation analysis of calcium channel function in active zones of motor nerve terminals.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- Monitoring presynaptic calcium dynamics with membrane-impermeant fluorescent indicators in motor nerve endings.Biophysical reviews · 2025Review
- Ageing-induced weakness of mouse NMJs is associated with reduced active zone density, synaptic event kinetics and presynaptic calcium entry.The Journal of physiology · 2025Article
- Article
- Neuromuscular Active Zone Structure and Function in Healthy and Lambert-Eaton Myasthenic Syndrome States.Biomolecules · 2022Review
- Similarity and Diversity of Presynaptic Molecules at Neuromuscular Junctions and Central Synapses.Biomolecules · 2022Review
- Diverse organization of voltage-gated calcium channels at presynaptic active zones.Frontiers in synaptic neuroscience · 2022Review
- Mechanisms controlling the trafficking, localization, and abundance of presynaptic CaFrontiers in molecular neuroscience · 2022Review
- Microphysiological Modeling of the Structure and Function of Neuromuscular Transmitter Release Sites.Frontiers in synaptic neuroscience · 2022Review
- The Frog Motor Nerve Terminal Has Very Brief Action Potentials and Three Electrical Regions Predicted to Differentially Control Transmitter Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Endogenous Tagging Reveals Differential Regulation of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- The control of release probability at nerve terminals.Nature reviews. Neuroscience · 2019Review
- Presynaptic mechanisms controlling calcium-triggered transmitter release at the neuromuscular junction.Current opinion in physiology · 2018Article
- Transmitter release site organization can predict synaptic function at the neuromuscular junction.Journal of neurophysiology · 2018Article
- Calcium dependence of spontaneous neurotransmitter release.Journal of neuroscience research · 2018Review
- Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.Neuroscience research · 2018Review
- Quantitative optical nanophysiology of CaNature communications · 2018Article
- Lambert-Eaton myasthenic syndrome: mouse passive-transfer model illuminates disease pathology and facilitates testing therapeutic leads.Annals of the New York Academy of Sciences · 2018Review
- Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction.Journal of visualized experiments : JoVE · 2017Article
- A stochastic model of active zone material mediated synaptic vesicle docking and priming at resting active zones.Scientific reports · 2017Article
- Synaptic Pathophysiology and Treatment of Lambert-Eaton Myasthenic Syndrome.Molecular neurobiology · 2015Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We used high-resolution fluorescence imaging and single-pixel optical fluctuation analysis to estimate the opening probability of individual voltage-gated calcium (Ca(2+)) channels during an action potential and the number of such Ca(2+) channels within active zones of frog neuromuscular junctions. Analysis revealed ∼36 Ca(2+) channels within each active zone, similar to the number of docked synaptic vesicles but far less than the total number of transmembrane particles reported based on freeze-fracture analysis (∼200-250). The probability that each channel opened during an action potential was only ∼0.2. These results suggest why each active zone averages only one quantal release event during every other action potential, despite a substantial number of docked vesicles. With sparse Ca(2+) channels and low opening probability, triggering of fusion for each vesicle is primarily controlled by Ca(2+) influx through individual Ca(2+) channels. In contrast, the entire synapse is highly reliable because it contains hundreds of active zones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.