Evidence map›Paper›PMID 21813665›Full record

ArticleClinical and vaccine immunology : CVI2011

A tripartite fusion, FaeG-FedF-LT(192)A2:B, of enterotoxigenic Escherichia coli (ETEC) elicits antibodies that neutralize cholera toxin, inhibit adherence of K88 (F4) and F18 fimbriae, and protect pigs against K88ac/heat-labile toxin infection.

Xiaosai Ruan, Mei Liu, Thomas A Casey, Weiping Zhang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and vaccine immunology : CVI, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 42 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Survey on resistance occurrence for F4Veterinary research communications · 2024
    Article
  5. The Diversity ofMicroorganisms · 2023
    Review
  6. Review on Preventive Measures to Reduce Post-Weaning Diarrhoea in Piglets.Animals : an open access journal from MDPI · 2022
    Review
  7. Article
  8. Article
  9. Pig vaccination strategies based on enterotoxigenic Escherichia coli toxins.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Xiaosai RuanVaccinology/Veterinary & Biomedical Sciences Department/The Center for Infectious Disease Research, Box 2157, South Dakota State University, Brookings, SD 57006, USA.
Mei Liu
Thomas A Casey
Weiping Zhang
South Dakota State University · USAgricultural Research Service · US

Funding

Determining vaccine candidacy of LT and STa toxoid fusions against enterotoxigeniR21AI083897 · NIAID · SOUTH DAKOTA STATE UNIVERSITY · PI ZHANG, WEIPING · 2009 to 2010
$368k
NIAID NIH HHS AI083897NIAID NIH HHS R21 AI083897
6 · The paper itself

Abstract

Enterotoxigenic Escherichia coli (ETEC) strains expressing K88 (F4) or F18 fimbriae and heat-labile (LT) and/or heat-stable (ST) toxins are the major cause of diarrhea in young pigs. Effective vaccines inducing antiadhesin (anti-K88 and anti-F18) and antitoxin (anti-LT and anti-ST) immunity would provide broad protection to young pigs against ETEC. In this study, we genetically fused nucleotides coding for peptides from K88ac major subunit FaeG, F18 minor subunit FedF, and LT toxoid (LT(192)) A2 and B subunits for a tripartite adhesin-adhesin-toxoid fusion (FaeG-FedF-LT(192)A2:B). This fusion was used for immunizations in mice and pigs to assess the induction of antiadhesin and antitoxin antibodies. In addition, protection by the elicited antiadhesin and antitoxin antibodies against a porcine ETEC strain was evaluated in a gnotobiotic piglet challenge model. The data showed that this FaeG-FedF-LT(192)A2:B fusion elicited anti-K88, anti-F18, and anti-LT antibodies in immunized mice and pigs. In addition, the anti-porcine antibodies elicited neutralized cholera toxin and inhibited adherence against both K88 and F18 fimbriae. Moreover, immunized piglets were protected when challenged with ETEC strain 30302 (K88ac/LT/STb) and did not develop clinical disease. In contrast, all control nonvaccinated piglets developed severe diarrhea and dehydration after being challenged with the same ETEC strain. This study clearly demonstrated that this FaeG-FedF-LT(192)A2:B fusion antigen elicited antibodies that neutralized LT toxin and inhibited the adherence of K88 and F18 fimbrial E. coli strains and that this fusion could serve as an antigen for vaccines against porcine ETEC diarrhea. In addition, the adhesin-toxoid fusion approach used in this study may provide important information for developing effective vaccines against human ETEC diarrhea.

Indexed as

Adhesins, BacterialAdhesins, Escherichia coliAnimalsAntibodies, BacterialAntigens, BacterialAntitoxinsBacterial ToxinsCholera ToxinDiarrheaEnterotoxigenic Escherichia coliEnterotoxinsEscherichia coli InfectionsEscherichia coli ProteinsEscherichia coli VaccinesFimbriae ProteinsMiceAdhesins, BacterialAdhesins, Escherichia coliAntibodies, BacterialAntigens, BacterialAntitoxinsBacterial ToxinsCholera ToxinEnterotoxinsEscherichia coli ProteinsEscherichia coli VaccinesFaeG protein, E coliFedA protein, E coliFedF protein, E coliFimbriae Proteinsheat-labile enterotoxin, E coliK88 antigen, E coliRecombinant Fusion Proteins

Identifiers

PMID21813665
PMCPMC3187021
OpenAlexW2104467918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.