ArticleDevelopment (Cambridge, England)2011
Complex interactions between cis-regulatory modules in native conformation are critical for Drosophila snail expression.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed.
- Expanding the fly eye gene regulatory network: From Drosophila to the hoverfly Episyrphus balteatus.PLoS genetics · 2026Article
- Enhancer Trajectories in Lineage Commitment: Regulatory Logic of States and Cooperation.Biomolecules · 2026Review
- Coordinated active repression operates via transcription factor cooperativity and multiple inactive promoter states in a developing organism.Nature communications · 2025Article
- The proximal enhancer of the snail gene mediates negative autoregulatory feedback in Drosophila melanogaster.Genetics · 2025Article
- The RNA Revolution in the Central Molecular Biology Dogma Evolution.International journal of molecular sciences · 2024Review
- Two sequential gene expression programs bridged by cell division support long-distance collective cell migration.Development (Cambridge, England) · 2024Article
- Modulation of protein-DNA binding reveals mechanisms of spatiotemporal gene control in earlyeLife · 2023Article
- Shadow enhancers mediate trade-offs between transcriptional noise and fidelity.PLoS computational biology · 2023Article
- Specification of the endocrine primordia controlling insect moulting and metamorphosis by the JAK/STAT signalling pathway.PLoS genetics · 2022Article
- Sex-specific evolution of a Drosophila sensory system via interacting cis- and trans-regulatory changes.Evolution & development · 2022Article
- Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos.Nucleic acids research · 2022Article
- A dual role for DNA binding by Runt in activation and repression ofMolecular biology of the cell · 2021Article
- Temporal integration of inductive cues on the way to gastrulation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Enhancer redundancy in development and disease.Nature reviews. Genetics · 2021Review
- Cooperative binding between distant transcription factors is a hallmark of active enhancers.Molecular cell · 2021Article
- Dynamic patterning by morphogens illuminated by cis-regulatory studies.Development (Cambridge, England) · 2021Review
- Shadow enhancers can suppress input transcription factor noise through distinct regulatory logic.eLife · 2020Article
- Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron.Genes & development · 2020Article
- Identification ofMolecules and cells · 2020Article
- Snail synchronizes endocycling in a TOR-dependent manner to coordinate entry and escape from endoreplication pausing during the Drosophila critical weight checkpoint.PLoS biology · 2020Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
It has been shown in several organisms that multiple cis-regulatory modules (CRMs) of a gene locus can be active concurrently to support similar spatiotemporal expression. To understand the functional importance of such seemingly redundant CRMs, we examined two CRMs from the Drosophila snail gene locus, which are both active in the ventral region of pre-gastrulation embryos. By performing a deletion series in a ∼25 kb DNA rescue construct using BAC recombineering and site-directed transgenesis, we demonstrate that the two CRMs are not redundant. The distal CRM is absolutely required for viability, whereas the proximal CRM is required only under extreme conditions such as high temperature. Consistent with their distinct requirements, the CRMs support distinct expression patterns: the proximal CRM exhibits an expanded expression domain relative to endogenous snail, whereas the distal CRM exhibits almost complete overlap with snail except at the anterior-most pole. We further show that the distal CRM normally limits the increased expression domain of the proximal CRM and that the proximal CRM serves as a `damper' for the expression levels driven by the distal CRM. Thus, the two CRMs interact in cis in a non-additive fashion and these interactions may be important for fine-tuning the domains and levels of gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.