ArticlePloS one2011
Expression of the lantibiotic mersacidin in Bacillus amyloliquefaciens FZB42.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 67 citations in OpenAlex.
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- Biochemical characterisation and production kinetics of high molecular-weight (HMW) putative antibacterial proteins of insect pathogenic Brevibacillus laterosporus isolates.BMC microbiology · 2024Article
- Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield.Applied microbiology and biotechnology · 2023Review
- Article
- Use of the mCherry fluorescent protein to optimize the expression of class I lanthipeptides in Escherichia coli.Microbial cell factories · 2023Article
- Mutational Studies of the Mersacidin Leader Reveal the Function of Its Unique Two-Step Leader Processing Mechanism.ACS synthetic biology · 2022Article
- Biological Membrane-Penetrating Peptides: Computational Prediction and Applications.Frontiers in cellular and infection microbiology · 2022Review
- Bioinoculants-Natural Biological Resources for Sustainable Plant Production.Microorganisms · 2021Review
- Microbial production of small peptide: pathway engineering and synthetic biology.Microbial biotechnology · 2021Review
- Heterologous Expression of Mersacidin inACS synthetic biology · 2021Article
- Simultaneous Production of Multiple Antimicrobial Compounds byFrontiers in microbiology · 2021Article
- Characterization of Leader Processing Shows That Partially Processed Mersacidin Is Activated by AprE After Export.Frontiers in microbiology · 2021Article
- Lesson from Ecotoxicity: Revisiting the Microbial Lipopeptides for the Management of Emerging Diseases for Crop Protection.International journal of environmental research and public health · 2020Review
- Comparative Genomics Analysis Provides New Strategies for Bacteriostatic Ability ofFrontiers in microbiology · 2020Article
- Surfactin Like Broad Spectrum Antimicrobial Lipopeptide Co-produced With Sublancin FromFrontiers in microbiology · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Draft Genome Sequence of Bacillus velezensis PEBA20, a Strain with a Plant Growth-Promoting Effect and Biocontrol Potential.Genome announcements · 2018Article
- Effects of dietaryJournal of animal science and biotechnology · 2018Article
- Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species.BMC genomics · 2016Article
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledLantibiotics are small peptide antibiotics that contain the characteristic thioether amino acids lanthionine and methyllanthionine. As ribosomally synthesized peptides, lantibiotics possess biosynthetic gene clusters which contain the structural gene (lanA) as well as the other genes which are involved in lantibiotic modification (lanM, lanB, lanC, lanP), regulation (lanR, lanK), export (lanT(P)) and immunity (lanEFG). The lantibiotic mersacidin is produced by Bacillus sp. HIL Y-85,54728, which is not naturally competent. METHODOLOGY/PRINCIPAL
findingsThe aim of these studies was to test if the production of mersacidin could be transferred to a naturally competent Bacillus strain employing genomic DNA of the producer strain. Bacillus amyloliquefaciens FZB42 was chosen for these experiments because it already harbors the mersacidin immunity genes. After transfer of the biosynthetic part of the gene cluster by competence transformation, production of active mersacidin was obtained from a plasmid in trans. Furthermore, comparison of several DNA sequences and biochemical testing of B. amyloliquefaciens FZB42 and B. sp. HIL Y-85,54728 showed that the producer strain of mersacidin is a member of the species B. amyloliquefaciens. CONCLUSIONS/SIGNIFICANCE: The lantibiotic mersacidin can be produced in B. amyloliquefaciens FZB42, which is closely related to the wild type producer strain of mersacidin. The new mersacidin producer strain enables us to use the full potential of the biosynthetic gene cluster for genetic manipulation and downstream modification approaches.
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