ArticlePLoS genetics2011
Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Temporal analysis of two inducible human genes reveals post-transcriptional H3K4me3 deposition.Life science alliance · 2026Article
- Nucleosomes represent a crucial target for the intra-S phase checkpoint in response to replication stress.Science advances · 2025Article
- RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation.Learning & memory (Cold Spring Harbor, N.Y.) · 2024Article
- Ubiquitination of Histone H2B by Proteasome Subunit RPT6 Controls Histone Methylation Chromatin Dynamics During Memory Formation.Biological psychiatry · 2021Article
- RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.Nucleic acids research · 2019Article
- Bre1-dependent H2B ubiquitination promotes homologous recombination by stimulating histone eviction at DNA breaks.Nucleic acids research · 2018Article
- Modes of Interaction of KMT2 Histone H3 Lysine 4 Methyltransferase/COMPASS Complexes with Chromatin.Cells · 2018Review
- H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation.Nucleic acids research · 2016Article
- Histone modifications as regulators of life and death inMicrobial cell (Graz, Austria) · 2015Review
- Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Review
- A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.Epigenetics & chromatin · 2015Article
- Epigenetic mechanisms of memory formation and reconsolidation.Neurobiology of learning and memory · 2014Review
- Epigenetic dysregulation by nickel through repressive chromatin domain disruption.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Centromeric histone H2B monoubiquitination promotes noncoding transcription and chromatin integrity.Nature structural & molecular biology · 2014Article
- RNA profiles of porcine embryos during genome activation reveal complex metabolic switch sensitive to in vitro conditions.PloS one · 2013Article
- Natural variation of H3K27me3 distribution between two Arabidopsis accessions and its association with flanking transposable elements.Genome biology · 2012Article
- R-loops and genomic instability in Bre1 (RNF20/40)-deficient cells.Cell cycle (Georgetown, Tex.) · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation. The maintenance of chromatin domain integrity can require the setting of boundaries. The HS4 insulator element marks the 3' boundary of a heterochromatin region located upstream of the chicken β-globin gene cluster. Here we show that HS4 recruits the E3 ligase RNF20/BRE1A to mediate H2B mono-ubiquitination (H2Bub1) at this insulator. Knockdown experiments show that RNF20 is required for H2Bub1 and processive H3K4 methylation. Depletion of RNF20 results in a collapse of the active histone modification signature at the HS4 chromatin boundary, where H2Bub1, H3K4 methylation, and hyperacetylation of H3, H4, and H2A.Z are rapidly lost. A remarkably similar set of events occurs at the HSA/HSB regulatory elements of the FOLR1 gene, which mark the 5' boundary of the same heterochromatin region. We find that persistent H2Bub1 at the HSA/HSB and HS4 elements is required for chromatin boundary integrity. The loss of boundary function leads to the sequential spreading of H3K9me2, H3K9me3, and H4K20me3 over the entire 50 kb FOLR1 and β-globin region and silencing of FOLR1 expression. These findings show that the HSA/HSB and HS4 boundary elements direct a cascade of active histone modifications that defend the FOLR1 and β-globin gene loci from the pervasive encroachment of an adjacent heterochromatin domain. We propose that many gene loci employ H2Bub1-dependent boundaries to prevent heterochromatin spreading.
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