ArticleJournal of biomedicine & biotechnology2011
In vitro culture conditions for maintaining a complex population of human gastrointestinal tract microbiota.
Article in Journal of biomedicine & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- The impact of coffee on gut microbial structure based on in vitro fecal incubation system.Food science and biotechnology · 2025Article
- Dissecting the human gut microbiome to better decipher drug liability: A once-forgotten organ takes center stage.Journal of advanced research · 2023Review
- Butyrogenic effect of galactosyl and mannosyl carbohydrates and their regulation on piglet intestinal microbiota.Applied microbiology and biotechnology · 2023Article
- Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse Model to Study Microbiome Function.mSystems · 2022Article
- Host cells subdivide nutrient niches into discrete biogeographical microhabitats for gut microbes.Cell host & microbe · 2022Article
- Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model.Antibiotics (Basel, Switzerland) · 2021Article
- Optimization of probiotic therapeutics using machine learning in an artificial human gastrointestinal tract.Scientific reports · 2021Article
- An in vitro model maintaining taxon-specific functional activities of the gut microbiome.Nature communications · 2019Article
- Dose-Dependent Effects of Aloin on the Intestinal Bacterial Community Structure, Short Chain Fatty Acids Metabolism and Intestinal Epithelial Cell Permeability.Frontiers in microbiology · 2019Article
- Article
- An in vitro study to assess the impact of tetracycline on the human intestinal microbiome.Anaerobe · 2018Article
- Responses of intestinal virome to silver nanoparticles: safety assessment by classical virology, whole-genome sequencing and bioinformatics approaches.International journal of nanomedicine · 2018Article
- Capturing the diversity of the human gut microbiota through culture-enriched molecular profiling.Genome medicine · 2016Article
- Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating.Scientific reports · 2015Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A stable intestinal microbiota is important in maintaining human physiology and health. Although there have been a number of studies using in vitro and in vivo approaches to determine the impact of diet and xenobiotics on intestinal microbiota, there is no consensus for the best in vitro culture conditions for growth of the human gastrointestinal microbiota. To investigate the dynamics and activities of intestinal microbiota, it is important for the culture conditions to support the growth of a wide range of intestinal bacteria and maintain a complex microbial community representative of the human gastrointestinal tract. Here, we compared the bacterial community in three culture media: brain heart infusion broth and high- and low-carbohydrate medium with different growth supplements. The bacterial community was analyzed using denaturing gradient gel electrophoresis (DGGE), pyrosequencing and real-time PCR. Based on the molecular analysis, this study indicated that the 3% fecal inoculum in low-concentration carbohydrate medium with 1% autoclaved fecal supernatant provided enhanced growth conditions to conduct in vitro studies representative of the human intestinal microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.