ArticleAnalytical chemistry2011
Imaging single-cell signaling dynamics with a deterministic high-density single-cell trap array.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed.
- Self-Regulated Selective Surface Coating Enables Confinement of Adherent Cells in Closed Microfluidic Arrays.Analytical chemistry · 2025Article
- The clock in growing hyphae and their synchronization in Neurospora crassa.Communications biology · 2024Article
- Single-cell trapping and retrieval in open microfluidics.iScience · 2023Article
- Single-Cell Isolation Microfluidic Chip Based on Thermal Bubble Micropump Technology.Sensors (Basel, Switzerland) · 2023Article
- Cell pairing for biological analysis in microfluidic devices.Biomicrofluidics · 2022Review
- Droplet microfluidics for functional temporal analysis and cell recovery on demand using microvalves: application in immunotherapies for cancer.Lab on a chip · 2022Article
- Massively parallel quantification of phenotypic heterogeneity in single-cell drug responses.Science advances · 2021Article
- High-Throughput Cell Trapping in the Dentate Spiral Microfluidic Channel.Micromachines · 2021Article
- High-Temporal-Resolution smFISH Method for Gene Expression Studies inAnalytical chemistry · 2021Article
- Facile Production of Large-Area Cell Arrays Using Surface-Assembled Microdroplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates.The Analyst · 2020Article
- A Precise Microfluidic Assay in Single-Cell Profile for Screening of Transient Receptor Potential Channel Modulators.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic systems for hydrodynamic trapping of cells and clusters.Biomicrofluidics · 2020Review
- smFISH in chips: a microfluidic-based pipeline to quantify in situ gene expression in whole organisms.Lab on a chip · 2020Article
- Neutrophil-targeted, protease-activated pulmonary drug delivery blocks airway and systemic inflammation.JCI insight · 2019Article
- Dynamic Mitochondrial Migratory Features Associated with Calcium Responses during T Cell Antigen Recognition.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Transport of dynamic biochemical signals in a microfluidic single cell trapping channel with varying cross-sections.The European physical journal. E, Soft matter · 2019Article
- Microfluidic Based Optical Microscopes on Chip.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Review
- A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins.Scientific reports · 2018Article
- An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood.Biomicrofluidics · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations, the analysis of which could lead to a better understanding of cellular processes and drug responses. Current technologies are limited in their throughput, resolution (in space, time, and tracking individual cells instead of population average) and the ability to control cellular environment. A few microfluidic tools have been developed to trap and image cells; however, in most designs available to date, there is a compromise among loading efficiency, speed, the ability to trap single cells, and density or number of trapped cells. To meet the needs of single-cell imaging studies, we developed a microfluidic platform for high-throughput capture and imaging of thousands of single cells. The optimized trapping mechanism enables 95% of the traps to be occupied with single cells, with a trap density of 860 traps/mm(2). The dense array allows up to 800 cells to be imaged simultaneously with a 4x objective and a typical camera setup. Capture occurs with low shear and 94% viability after 24 h. This platform is compatible with other upstream microfluidic components for complex cell stimulation patterns, and we show here the ability to measure heterogeneity in calcium oscillatory behavior in genetically identical cells and monitor kinetic cellular response to chemical stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.