Evidence map›Paper›PMID 21809317›Full record

ArticleCurrent protocols in immunology2011

Unbiased molecular analysis of T cell receptor expression using template-switch anchored RT-PCR.

Máire F Quigley, Jorge R Almeida, David A Price, Daniel C Douek

Open access · greenAbstract read
In one paragraph

Article in Current protocols in immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 85 citations in OpenAlex.

  1. Trial
  2. Review
  3. Article
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  5. Article
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  9. Article
  10. Article
  11. Article
  12. CD4Cell reports · 2020
    Article
  13. Circulating gluten-specific, but not CMV-specific, CD39Clinical & translational immunology · 2020
    Article
  14. Interstitial-resident memory CD8The Journal of experimental medicine · 2019
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. The Human CD4Journal of virology · 2019
    Article
  20. Memory CD4Nature immunology · 2019
    Article

1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Máire F QuigleyVaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Jorge R Almeida
David A Price
Daniel C Douek
National Institute of Allergy and Infectious Diseases · USNational Institutes of Health · US

Funding

Host Genomic Factors in Infectious Disease Susceptibility ZIAAI005066 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DOUEK, DANIEL CESAR · 2009 to 2025
$17.9M
T Cell Immune Responses To HIV And Other PathogensZIAAI005033 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DOUEK, DANIEL CESAR · 2009 to 2025
$11.4M
Intramural NIH HHS ZIA AI005066Medical Research Council G0501963
6 · The paper itself

Abstract

A detailed knowledge of the principles that guide clonal selection within the memory and effector T cell pools is essential to further our understanding of the factors that influence effective T cell-mediated immunity and has direct implications for the rational design of vaccines and immunotherapies. This unit provides methods for the unbiased quantification and characterization of all expressed T cell receptor (TCR) gene products within any defined T cell population. The approach is based on a template-switch anchored reverse transcription-polymerase chain reaction (RT-PCR) and is optimized for the analysis of antigen-specific T cells isolated directly ex vivo.

Indexed as

Genes, T-Cell ReceptorPolymerase Chain ReactionAnimalsClone CellsHumansImmunity, CellularPathology, MolecularT-Cell Antigen Receptor SpecificityTemplates, GeneticT-Lymphocytes

Identifiers

PMID21809317
PMCPMC3154014
OpenAlexW1855466940

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.