ArticleMolecular & cellular proteomics : MCP2011
The RNA-binding protein Rrm4 is essential for efficient secretion of endochitinase Cts1.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Dissecting the RNA-binding capacity of the multi-RRM protein Rrm4 essential for endosomal mRNA transport.Nucleic acids research · 2026Article
- Vesicle-coupled mRNA transport and translation govern intracellular organelle networking.EMBO reports · 2026Review
- Silencing a Chitinase Gene,International journal of molecular sciences · 2023Article
- Post-transcriptional control of fungal cell wall synthesis.Cell surface (Amsterdam, Netherlands) · 2022Article
- Linking transport and translation of mRNAs with endosomes and mitochondria.EMBO reports · 2021Review
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- A Novel Potent Carrier for Unconventional Protein Export inFrontiers in cell and developmental biology · 2021Article
- Article
- A Novel Factor Essential for Unconventional Secretion of Chitinase Cts1.Frontiers in microbiology · 2020Article
- N-glycosylation of the protein disulfide isomerase Pdi1 ensures full Ustilago maydis virulence.PLoS pathogens · 2019Article
- The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.EMBO reports · 2019Article
- A Potential Lock-Type Mechanism for Unconventional Secretion in Fungi.International journal of molecular sciences · 2019Review
- The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs.EMBO reports · 2019Article
- Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures.Microbiology and molecular biology reviews : MMBR · 2018Review
- Application of the pathogen Trojan horse approach in maize (Zea mays).Plant signaling & behavior · 2018Article
- Messenger RNA transport in the opportunistic fungal pathogen Candida albicans.Current genetics · 2017Review
- Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.International journal of molecular sciences · 2017Article
- The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.PLoS genetics · 2017Article
- Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions.Journal of visualized experiments : JoVE · 2016Article
- Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions.Current genetics · 2016Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
Long-distance transport of mRNAs is crucial in determining spatio-temporal gene expression in eukaryotes. The RNA-binding protein Rrm4 constitutes a key component of microtubule-dependent mRNA transport in filaments of Ustilago maydis. Although a number of potential target mRNAs could be identified, cellular processes that depend on Rrm4-mediated transport remain largely unknown. Here, we used differential proteomics to show that ribosomal, mitochondrial, and cell wall-remodeling proteins, including the bacterial-type endochitinase Cts1, are differentially regulated in rrm4Δ filaments. In vivo UV crosslinking and immunoprecipitation and fluorescence in situ hybridization revealed that cts1 mRNA represents a direct target of Rrm4. Filaments of cts1Δ mutants aggregate in liquid culture suggesting an altered cell surface. In wild type cells Cts1 localizes predominantly at the growth cone, whereas it accumulates at both poles in rrm4Δ filaments. The endochitinase is secreted and associates most likely with the cell wall of filaments. Secretion is drastically impaired in filaments lacking Rrm4 or conventional kinesin Kin1 as well as in filaments with disrupted microtubules. Thus, Rrm4-mediated mRNA transport appears to be essential for efficient export of active Cts1, uncovering a novel molecular link between mRNA transport and the mechanism of secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.