ArticleProceedings of the National Academy of Sciences of the United States of America2011
A bacterial process for selenium nanosphere assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 191 citations in OpenAlex.
- From selenite to selenium nanoparticle: comparative biosynthetic mechanisms across Bacillus and a roadmap for Bacillus licheniformis.World journal of microbiology & biotechnology · 2026Review
- Genomic Insights Into the Multimetal Resilience and Biofilm-Templated Nanorod Biosynthesis of Stenotrophomonas bentonitica BII-R7: Bioremediation and Green Nanotechnology Implications.Microbial biotechnology · 2026Article
- Green synthesis of selenium nanoparticles using Bacillus sp. strain STG-83: optimization, characterization, and prospects for cancer radiosensitization.Scientific reports · 2026Article
- Preparation, Characteristics, Antioxidant and Anti-Inflammatory Activity of SeNPs and ZnO-enriched Limosilactobacillus reuteri LR1.Probiotics and antimicrobial proteins · 2026Article
- Biosynthesis and Export of Membrane-Enveloped Selenium Nanoparticles byEnvironmental science & technology · 2026Article
- Subcellular Mechanisms of Se(IV) Reduction in Stenotrophomonas bentonitica: Linking Environmental Detoxification and Antimicrobial Activity of Se Nanostructures Within a Circular Economy Framework.Microbial biotechnology · 2025Article
- Bioremediation and optimization of selenate reduction by a novel Bacillus cabrialesii strain (Se1) producing red selenium nanoparticles with potential antibacterial activity.BMC microbiology · 2025Article
- Tellurite and selenite processing by tellurite resistant marine microbes.Applied and environmental microbiology · 2025Article
- Nanoprobe synchrotron X-ray fluorescence microscopy reveals selenium-rich spherical structure in mouse retinal pigment epithelium.Scientific reports · 2025Article
- Identification of a multi-modal mechanism for Se(VI) reduction and Se(0) allotropic transition by Stenotrophomonas bentonitica.Environmental science and pollution research international · 2025Article
- Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization.NPJ biofilms and microbiomes · 2025Article
- Microbial Nanoparticles in Biological Plant Protection.International journal of molecular sciences · 2025Review
- Clinical and mechanistic insights into biomedical application of Se-enriched probiotics and biogenic selenium nanoparticles.Biotechnology letters · 2025Review
- Biosynthesis of selenium nanoparticles as a potential therapeutic agent in breast cancer: G2/M arrest and apoptosis induction.Toxicology reports · 2024Article
- Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu fromJournal of Zhejiang University. Science. B · 2024Article
- Electroactive Brevundimonas diminuta consortium mediated selenite bioreduction, biogenesis of selenium nanoparticles and bio-electricity generation.Journal of nanobiotechnology · 2024Article
- Review
- Selenium Nanoparticles: Green Synthesis and Biomedical Application.Molecules (Basel, Switzerland) · 2023Review
- A Comparative Study of the Synthesis and Characterization of Biogenic Selenium Nanoparticles by Two Contrasting Endophytic Selenobacteria.Microorganisms · 2023Article
- Selenite Bioremediation by Food-Grade Probiotic Lactobacillus casei ATCC 393: Insights from Proteomics Analysis.Microbiology spectrum · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
During selenate respiration by Thauera selenatis, the reduction of selenate results in the formation of intracellular selenium (Se) deposits that are ultimately secreted as Se nanospheres of approximately 150 nm in diameter. We report that the Se nanospheres are associated with a protein of approximately 95 kDa. Subsequent experiments to investigate the expression and secretion profile of this protein have demonstrated that it is up-regulated and secreted in response to increasing selenite concentrations. The protein was purified from Se nanospheres, and peptide fragments from a tryptic digest were used to identify the gene in the draft T. selenatis genome. A matched open reading frame was located, encoding a protein with a calculated mass of 94.5 kDa. N-terminal sequence analysis of the mature protein revealed no cleavable signal peptide, suggesting that the protein is exported directly from the cytoplasm. The protein has been called Se factor A (SefA), and homologues of known function have not been reported previously. The sefA gene was cloned and expressed in Escherichia coli, and the recombinant His-tagged SefA purified. In vivo experiments demonstrate that SefA forms larger (approximately 300 nm) Se nanospheres in E. coli when treated with selenite, and these are retained within the cell. In vitro assays demonstrate that the formation of Se nanospheres upon the reduction of selenite by glutathione are stabilized by the presence of SefA. The role of SefA in selenium nanosphere assembly has potential for exploitation in bionanomaterial fabrication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.