ArticleTalanta2011
Highly sensitive disposable nucleic acid biosensors for direct bioelectronic detection in raw biological samples.
Article in Talanta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 52 citations in OpenAlex.
- Strategic Detection ofBiosensors · 2025Review
- Investigating electrochemical deposition of gold on commercial off-the-shelf 3-D printing materials towards developing sensing applications.RSC advances · 2022Article
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- 3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection ofSensors (Basel, Switzerland) · 2020Article
- Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices.Sensors (Basel, Switzerland) · 2020Review
- Antifouling (Bio)materials for Electrochemical (Bio)sensing.International journal of molecular sciences · 2019Review
- Poly-L-lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection.Electroanalysis · 2018Article
- Dithiothreitol-Regulated Coverage of Oligonucleotide-Modified Gold Nanoparticles To Achieve Optimized Biosensor Performance.ACS applied materials & interfaces · 2018Article
- A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis.SLAS technology · 2017Article
- Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application.Sensors (Basel, Switzerland) · 2016Review
- Evaluation of the Spectral Response of Functionalized Silk Inverse Opals as Colorimetric Immunosensors.ACS applied materials & interfaces · 2016Article
- Rapid quantitative detection of Brucella melitensis by a label-free impedance immunosensor based on a gold nanoparticle-modified screen-printed carbon electrode.Sensors (Basel, Switzerland) · 2013Article
- Bacterial detection & identification using electrochemical sensors.Journal of visualized experiments : JoVE · 2013Article
- Rapid antimicrobial susceptibility testing by sensitive detection of precursor rRNA using a novel electrochemical biosensing platform.Antimicrobial agents and chemotherapy · 2013Article
- A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens.Talanta · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The development of rapid, low-cost and reliable diagnostic methods is crucial for the identification and treatment of many diseases. Screen-printed gold electrodes (Au/SPEs), coated with a ternary monolayer interface, involving hexanedithiol (HDT), a specific thiolated capture probe (SHCP), and 6-mercapto-1 hexanol (MCH) (SHCP/HDT/MCH) are shown here to offer direct and sensitive detection of nucleic acid hybridization events in untreated raw biological samples (serum, urine and crude bacterial lysate solutions). The composition of the ternary monolayer was modified and tailored to the surface of the Au/SPE. The resulting SHCP/HDT/MCH monolayer has demonstrated to be extremely useful for enhancing the performance of disposable nucleic acid sensors based on screen-printed electrodes. Compared to common SHCP/MCH binary interfaces, the new ternary self-assembled monolayer (SAM) resulted in a 10-fold improvement in the signal (S)-to-noise (N) ratio (S/N) for 1 nM target DNA. The SHCP/HDT/MCH-modified Au/SPEs allowed the direct quantification of the target DNA down to 25 pM (0.25 fmol) and 100 pM (1 fmol) in undiluted/untreated serum and urine samples, respectively, and of 16S rRNA Escherichia coli (E. coli) corresponding to 3000 CFU μL(-1) in raw cell lysate samples. The new SAM-coated screen-printed electrodes also displayed favorable non-fouling properties after a 24h exposure to raw human serum and urine samples, offering great promise as cost-effective nucleic acid sensors for a wide range of decentralized genetic tests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.