ArticleBiophysical journal2011
Imaging molecular transport across lipid bilayers.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- The Role of the Organic Moiety in the Diffusion and Transport of Carboxylates into Liposomes.Molecules (Basel, Switzerland) · 2024Article
- Dynamic Second Harmonic Imaging of Proton Translocation Through Water Needles in Lipid Membranes.Journal of the American Chemical Society · 2024Article
- How is Membrane Permeation of Small Ionizable Molecules Affected by Protonation Kinetics?The journal of physical chemistry. B · 2024Article
- Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors.Bio-protocol · 2023Article
- Oxylipin biosynthetic gene families of Cannabis sativa.PloS one · 2023Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Quantifying proton-induced membrane polarization in single biomimetic giant vesicles.Biophysical journal · 2022Article
- To Close or to Collapse: The Role of Charges on Membrane Stability upon Pore Formation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability.Communications biology · 2020Article
- Multi-phase Boltzmann weighting: accounting for local inhomogeneity in molecular simulations of water-octanol partition coefficients in the SAMPL6 challenge.Journal of computer-aided molecular design · 2020Article
- Influence of Ethyl Caproate on the Size of Lipid Vesicles and Yeast Cells.Biomimetics (Basel, Switzerland) · 2020Article
- Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport.Chemical science · 2020Article
- A microfluidic platform for the characterisation of membrane active antimicrobials.Lab on a chip · 2019Article
- The Effect of Buffers on Weak Acid Uptake by Vesicles.Biomolecules · 2019Article
- Opsin 1 and Opsin 2 of the Corn Smut FungusFrontiers in microbiology · 2019Article
- Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures.ChemistryOpen · 2018Article
- Visualization and quantification of transmembrane ion transport into giant unilamellar vesicles.Angewandte Chemie (International ed. in English) · 2015Article
- Low levels of lipid oxidation radically increase the passive permeability of lipid bilayers.Soft matter · 2015Article
- Optical stretching as a tool to investigate the mechanical properties of lipid bilayers.RSC advances · 2013Article
- Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore.Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Low-molecular-weight carboxylic acids have many properties common to small molecule drugs. The transport of these acids across cell membranes has been widely studied, but these studies have produced wildly varying permeability values. Recent reports have even claimed that the transport behavior of these drugs is contrary to the rule of thumb called Overton's rule, which holds that more lipophilic molecules transport across lipid membranes more quickly. We used confocal microscopy to image the transport of carboxylic acids with different lipophilicities into a giant unilamellar lipid vesicle (GUV). Fluorescein-dextran, which acts as a pH-sensitive dye, was encapsulated in the GUV to trace the transport of acid. The GUV was immobilized on the surface of a microfluidic channel by biotin-avidin binding. This microchannel allows the rapid and uniform exchange of the solution surrounding the GUV. Using a spinning-disk confocal microscope, the entire concentration field is captured in a short (<100 ms) exposure. Results show that more lipophilic acids cross the bilayer more quickly. A finite difference model was developed to simulate the experimental process and derive permeabilities. The permeabilities change with the same trend as literature oil-water partition coefficients, demonstrating that Overton's rule applies to this class of molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.