ArticleBMC research notes2011
Selection of suitable reference genes for quantitative real-time polymerase chain reaction in human meningiomas and arachnoidea.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
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- Optimization of internal reference genes for qPCR in human pancreatic cancer research.Translational cancer research · 2020Article
- An integrated genomic analysis of anaplastic meningioma identifies prognostic molecular signatures.Scientific reports · 2018Article
- Predictive value of PD-L1 based on mRNA level in the treatment of stage IV melanoma with ipilimumab.Journal of cancer research and clinical oncology · 2017Article
- Aberrant DNA methylation of alternative promoter of DLC1 isoform 1 in meningiomas.Journal of neuro-oncology · 2016Article
- Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas).PeerJ · 2016Article
- Identification of Reference Genes for Quantitative Real Time PCR Assays in Aortic Tissue of Syrian Hamsters with Bicuspid Aortic Valve.PloS one · 2016Article
- Endogenous Reference Genes for Gene Expression Studies on Bicuspid Aortic Valve Associated Aortopathy in Humans.PloS one · 2016Article
- Identification of stable reference genes for gene expression analysis of three-dimensional cultivated human bone marrow-derived mesenchymal stromal cells for bone tissue engineering.Tissue engineering. Part C, Methods · 2015Article
- Impaired TrkB receptor signaling underlies corticostriatal dysfunction in Huntington's disease.Neuron · 2014Article
- Impaired striatal function in Huntington's disease is due to aberrant p75NTR signaling.Rare diseases (Austin, Tex.) · 2014Article
- Selection of reference genes for quantitative real time PCR (qPCR) assays in tissue from human ascending aorta.PloS one · 2014Article
- Reference gene selection for quantitative PCR studies in sheep neutrophils.International journal of molecular sciences · 2013Article
- Transcriptome Analysis of Spermophilus lateralis and Spermophilus tridecemlineatus Liver Does Not Suggest the Presence of Spermophilus-Liver-Specific Reference Genes.ISRN bioinformatics · 2013Article
- Vascular endothelial growth factor signals through platelet-derived growth factor receptor β in meningiomas in vitro.British journal of cancer · 2012Article
- Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper.International journal of legal medicine · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
findingsAt first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method. Reference genes were ranked according to an integration tool for analyzing reference genes expression based on those four algorithms. Eight highest ranked reference genes (CASC3, EIF2B1, IPO8, MRPL19, PGK1, POP4, PPIA, and RPL37A) plus GAPDH and ACTB were then analyzed in 35 meningiomas, arachnoidea, dura mater and normal brain. NormFinder and Bestkeeper-1 identified RPL37A as the most stable expressed gene in meningiomas and their normal control tissue. NormFinder also determined the best combination of genes: RPL37A and EIF2B1. Commonly used reference genes GAPDH and ACTB were considered least stable genes. The critical influence of reference genes on qPCR data analysis is shown for VEGFA transcription patterns.
backgroundIn meningiomas quantitative real-time reverse transcription-polymerase chain reaction (qPCR) is most frequently used for accurate determination of gene expression using various reference genes. Although meningiomas are a heterogeneous group of tissue, no data have been reported to validate reference genes for meningiomas and their control tissues.
conclusionsRPL37A is the optimal single reference gene for normalization of gene expression in meningiomas and their control tissues, although the use of the combination of RPL37A and EIF2B1 would provide more stable results.
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Registered trials
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