ArticleBMC genomics2011
Large-scale RNAi screen of G protein-coupled receptors involved in larval growth, molting and metamorphosis in the red flour beetle.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 104 citations in OpenAlex.
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- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies,International journal of molecular sciences · 2024Article
- Evolutionary conserved peptide and glycoprotein hormone-like neuroendocrine systems in C. elegans.Molecular and cellular endocrinology · 2024Article
- Genetic independence between traits separated by metamorphosis is widespread but varies with biological function.Proceedings. Biological sciences · 2023Article
- Identification of three elevenin receptors and roles of elevenin disulfide bond and residues in receptor activation in Aplysia californica.Scientific reports · 2023Article
- Characterisation of Elevenin-Vc1 from the Venom ofMarine drugs · 2023Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Persistent Parental RNAi in the BeetleAdvanced genetics (Hoboken, N.J.) · 2022Article
- Homology Modeling and Molecular Docking Approaches for the Proposal of Novel Insecticides against the African Malaria Mosquito (Molecules (Basel, Switzerland) · 2022Article
- Article
- G protein-coupled receptors that influence lifespan of human and animal models.Biogerontology · 2022Review
- Identification of suitable reference genes for expression profiling studies using qRT-PCR in an important insect pest, Maruca vitrata.Molecular biology reports · 2021Article
- Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp,Insects · 2021Article
- Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis).Marine biotechnology (New York, N.Y.) · 2021Article
- G-Protein Coupled Receptors (GPCRs) in Insects-A Potential Target for New Insecticide Development.Molecules (Basel, Switzerland) · 2021Review
- G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology.International journal of molecular sciences · 2021Review
- Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes.Insect biochemistry and molecular biology · 2020Article
- Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe G protein-coupled receptors (GPCRs) belong to the largest superfamily of integral cell membrane proteins and play crucial roles in physiological processes including behavior, development and reproduction. Because of their broad and diverse roles in cellular signaling, GPCRs are the therapeutic targets for many prescription drugs. However, there is no commercial pesticide targeting insect GPCRs. In this study, we employed functional genomics methods and used the red flour beetle, Tribolium castaneum, as a model system to study the physiological roles of GPCRs during the larval growth, molting and metamorphosis.
resultsA total of 111 non-sensory GPCRs were identified in the T. castaneum genome. Thirty-nine of them were not reported previously. Large-scale RNA interference (RNAi) screen was used to study the function of all these GPCRs during immature stages. Double-stranded RNA (dsRNA)-mediated knockdown in the expression of genes coding for eight GPCRs caused severe developmental arrest and ecdysis failure (with more than 90% mortality after dsRNA injection). These GPCRs include dopamine-2 like receptor (TC007490/D2R) and latrophilin receptor (TC001872/Cirl). The majority of larvae injected with TC007490/D2R dsRNA died during larval stage prior to entering pupal stage, suggesting that this GPCR is essential for larval growth and development.
conclusionsThe results from our study revealed the physiological roles of some GPCRs in T. castaneum. These findings could help in development of novel pesticides targeting these GPCRs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.