ArticleJournal of neurochemistry2011
Effects of chronic nicotine on heteromeric neuronal nicotinic receptors in rat primary cultured neurons.
Article in Journal of neurochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Cytotoxic and genotoxic evaluation of cotinine using human neuroblastoma cells (SH-SY5Y).Genetics and molecular biology · 2020Article
- Chronic Menthol Does Not Change Stoichiometry or Functional Plasma Membrane Levels of MouseMolecular pharmacology · 2019Article
- Serine residues in the α4 nicotinic acetylcholine receptor subunit regulate surface α4β2Biochemical pharmacology · 2019Article
- Nicotine and electronic cigarette (E-Cig) exposure decreases brain glucose utilization in ischemic stroke.Journal of neurochemistry · 2018Article
- Reduced α4 subunit expression in α4British journal of pharmacology · 2018Article
- Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs).PloS one · 2018Article
- The (Molecular pharmacology · 2017Article
- RNAi technology: a new platform for crop pest control.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Review
- Exposure to nicotine increases nicotinic acetylcholine receptor density in the reward pathway and binge ethanol consumption in C57BL/6J adolescent female mice.Brain research bulletin · 2016Article
- Impact of short access nicotine self-administration on expression of α4β2* nicotinic acetylcholine receptors in non-human primates.Psychopharmacology · 2016Article
- Smoking-Relevant Nicotine Concentration Attenuates the Unfolded Protein Response in Dopaminergic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- The nicotine metabolite, cotinine, alters the assembly and trafficking of a subset of nicotinic acetylcholine receptors.The Journal of biological chemistry · 2015Article
- AT-1001 Is a Partial Agonist with High Affinity and Selectivity at Human and Rat α3β4 Nicotinic Cholinergic Receptors.Molecular pharmacology · 2015Article
- Density of α4β2* nAChR on the surface of neurons is modulated by chronic antagonist exposure.Pharmacology research & perspectives · 2015Article
- Chronic nicotine attenuates phencyclidine-induced impulsivity in a mouse serial reaction time task.Behavioural brain research · 2014Article
- Nicotine exploits a COPI-mediated process for chaperone-mediated up-regulation of its receptors.The Journal of general physiology · 2014Article
- Towards the elements of successful insect RNAi.Journal of insect physiology · 2013Review
- Chronic sazetidine-A at behaviorally active doses does not increase nicotinic cholinergic receptors in rodent brain.The Journal of pharmacology and experimental therapeutics · 2012Article
- Regulation of the distribution and function of [(125)I]epibatidine binding sites by chronic nicotine in mouse embryonic neuronal cultures.The Journal of pharmacology and experimental therapeutics · 2012Article
- The duration of nicotine withdrawal-associated deficits in contextual fear conditioning parallels changes in hippocampal high affinity nicotinic acetylcholine receptor upregulation.Neuropharmacology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Nicotine increases the number of neuronal nicotinic acetylcholine receptors (nAChRs) in brain. This study investigated the effects of chronic nicotine treatment on nAChRs expressed in primary cultured neurons. In particular, we studied the chronic effects of nicotine exposure on the total density, surface expression and turnover rate of heteromeric nAChRs. The receptor density was measured by [¹²⁵I]epibatidine ([¹²⁵I]EB) binding. Untreated and nicotine-treated neurons were compared from several regions of embryonic (E19) rat brain. Twelve days of treatment with 10 μM nicotine produced a twofold up-regulation of nAChRs. Biotinylation and whole-cell binding studies indicated that up-regulation resulted from an increase in the number of cell surface receptors as well as intracellular receptors. nAChR subunit composition in cortical and hippocampal neurons was assessed by immunoprecipitation with subunit-selective antibodies. These neurons contain predominantly α4, β2 and α5 subunits, but α2, α3, α6 and β4 subunits were also detected. Chronic nicotine exposure yielded a twofold increase in the β2-containing receptors and a smaller up-regulation in the α4-containing nAChRs. To explore the mechanisms of up-regulation we investigated the effects of nicotine on the receptor turnover rate. We found that the turnover rate of surface receptors was > 2 weeks and chronic nicotine exposure had no effect on this rate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.