ArticleProteomics2011
Identification and validation of mouse sperm proteins correlated with epididymal maturation.
Article in Proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Seasonal adaptations in the ultrastructural and immunohistochemical characterization of the epididymal duct in Meriz bucks.Open veterinary journal · 2026Article
- Dynamic sperm proteome remodeling during epididymal maturation in a marsupial, Macropus giganteus†.Biology of reproduction · 2025Article
- Bifenthrin Diminishes Male Fertility Potential by Inducing Protein Defects in Mouse Sperm.Toxics · 2024Article
- Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation.The world journal of men's health · 2022Article
- Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters.Poultry science · 2022Article
- Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation.Journal of extracellular vesicles · 2021Article
- Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis).Molecular reproduction and development · 2021Article
- Circular RNAs fromScience advances · 2020Article
- The preconception environment and sperm epigenetics.Andrology · 2020Review
- Dynamics of HSPA1A and redox status in the spermatozoa and fluid from different segments of goat epididymis.Cell stress & chaperones · 2020Article
- Proteomic Signatures in Spermatozoa Reveal the Role of Paternal Factors in Recurrent Pregnancy Loss.The world journal of men's health · 2020Article
- Genome-wide recombination map construction from single individuals using linked-read sequencing.Nature communications · 2019Article
- Diversity of ejaculated sperm proteins in Moxotó bucks (Animal reproduction · 2018Article
- Molecular changes and signaling events occurring in spermatozoa during epididymal maturation.Andrology · 2017Review
- Gestational Exposure to Bisphenol A Affects the Function and Proteome Profile of F1 Spermatozoa in Adult Mice.Environmental health perspectives · 2017Article
- Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation.Scientific reports · 2016Article
- Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm.Open biology · 2015Article
- Thiol changes during epididymal maturation: a link to flagellar angulation in mouse spermatozoa?Andrology · 2014Article
- Male mice express spermatogenic cell-specific triosephosphate isomerase isozymes.Molecular reproduction and development · 2013Article
- Sperm proteomics: road to male fertility and contraception.International journal of endocrinology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sperm need to mature in the epididymis to become capable of fertilization. To understand the molecular mechanisms of mouse sperm maturation, we conducted a proteomic analysis using saturation dye labeling to identify proteins of caput and cauda epididymal sperm that exhibited differences in amounts or positions on two-dimensional gels. Of eight caput epididymal sperm-differential proteins, three were molecular chaperones and three were structural proteins. Of nine cauda epididymal sperm-differential proteins, six were enzymes of energy metabolism. To validate these proteins as markers of epididymal maturation, immunoblotting and immunofluorescence analyses were performed. During epididymal transit, heat shock protein 2 was eliminated with the cytoplasmic droplet and smooth muscle γ-actin exhibited reduced fluorescence from the anterior acrosome while the signal intensity of aldolase A increased, especially in the principal piece. Besides these changes, we observed protein spots, such as glutathione S-transferase mu 5 and the E2 component of pyruvate dehydrogenase complex, shifting to more basic isoelectric points, suggesting post-translational changes such dephosphorylation occur during epididymal maturation. We conclude that most caput epididymal sperm-differential proteins contribute to the functional modification of sperm structures and that many cauda epididymal sperm-differential proteins are involved in ATP production that promotes sperm functions such as motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.