ArticleStem cells (Dayton, Ohio)2011
Transcriptome analysis of neural progenitor cells by a genetic dual reporter strategy.
Article in Stem cells (Dayton, Ohio), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Role and Mechanisms of Tyro3 in Podocyte Biology and Glomerular Disease.Kidney diseases (Basel, Switzerland) · 2024Review
- Molecular divergence of mammalian astrocyte progenitor cells at early gliogenesis.Development (Cambridge, England) · 2022Article
- Neurotrophins Time Point Intervention after Traumatic Brain Injury: From Zebrafish to Human.International journal of molecular sciences · 2021Review
- TAM Signaling in the Nervous System.Brain plasticity (Amsterdam, Netherlands) · 2021Article
- Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.Science advances · 2019Article
- Alternative RNA Splicing Associated With Mammalian Neuronal Differentiation.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- The role of TAM family receptors and ligands in the nervous system: From development to pathobiology.Pharmacology & therapeutics · 2018Review
- Alternative Splicing in Neurogenesis and Brain Development.Frontiers in molecular biosciences · 2018Review
- Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.Nature communications · 2017Article
- Dynamics of RNA Polymerase II Pausing and Bivalent Histone H3 Methylation during Neuronal Differentiation in Brain Development.Cell reports · 2017Article
- Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected.Acta neuropathologica communications · 2017Article
- Protein S Negatively Regulates Neural Stem Cell Self-Renewal through Bmi-1 Signaling.Frontiers in molecular neuroscience · 2017Article
- Identification of a common mesenchymal stromal progenitor for the adult haematopoietic niche.Nature communications · 2016Article
- Prospective separation and transcriptome analyses of cortical projection neurons and interneurons based on lineage tracing by Tbr2 (Eomes)-GFP/Dcx-mRFP reporters.Developmental neurobiology · 2016Article
- Expression Analysis Highlights AXL as a Candidate Zika Virus Entry Receptor in Neural Stem Cells.Cell stem cell · 2016Article
- TAM receptor deficiency affects adult hippocampal neurogenesis.Metabolic brain disease · 2015Review
- Axl as a mediator of cellular growth and survival.Oncotarget · 2014Review
- TAM receptors support neural stem cell survival, proliferation and neuronal differentiation.PloS one · 2014Article
- TAM receptors affect adult brain neurogenesis by negative regulation of microglial cell activation.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Dynamics of 5-hydroxymethylcytosine and chromatin marks in Mammalian neurogenesis.Cell reports · 2013Article
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9 authors at 3 institutions in 1 country.
Funding
Abstract
Global analysis of stem/progenitor cells promises new insight into mechanisms that govern self-renewal and cellular potential, an unresolved question of stem/progenitor cell biology. Despite rapid advance of genome-wide profiling methods, the difficulty in cell purification remains a major challenge for global analysis of somatic stem/progenitor cells. Genetic tagging with a reporter provides a powerful tool for identification and isolation of a specific mature cell type; however, for stem/progenitor cells, reporter retention by progeny may be a concern for impurity. Here, we describe a genetic system combining a progenitor cell specific label with a second tag for marking differentiation. We present evidence that differential labeling of neural progenitor cells and their progeny enables prospective purification of these two cell types, whereas isolation based on a single marker compromises the purity of the intended progenitor population. Comparative expression profiling between the purified progenitors and progeny documents a neural progenitor cell transcriptome and uncovers an important role of Tyro3/Axl/Mer receptor tyrosine kinases in the maintenance of neural progenitor cells. This study establishes a general strategy for isolation of somatic stem/progenitor cells and provides a transcriptome database of neural progenitor cells useful for identification of causal factors of neural progenitor cell state, global dissection of epigenetic control of cellular potential, as well as for developing biomarkers or targets of brain cancer stem/initiating cells for therapeutic interventions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.