ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2011
ΦC31 integrase mediates efficient cassette exchange in the zebrafish germline.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Cp36 serine recombinase as a new tool for zebrafish transgenesis.bioRxiv : the preprint server for biology · 2026Article
- There and turn back again: the application of phage serine integrases in eukaryotic systems.Frontiers in bioengineering and biotechnology · 2025Review
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- The preoptic area and dorsal habenula jointly support homeostatic navigation in larval zebrafish.Current biology : CB · 2024Article
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- Spinal cord repair is modulated by the neurogenic factor Hb-egf under direction of a regeneration-associated enhancer.Nature communications · 2023Article
- Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.Genetics · 2022Article
- Article
- Review
- A new model army: Emerging fish models to study the genomics of vertebrate Evo-Devo.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2015Review
- Differential in vivo tumorigenicity of diverse KRAS mutations in vertebrate pancreas: A comprehensive survey.Oncogene · 2015Article
- Developmental enhancers revealed by extensive DNA methylome maps of zebrafish early embryos.Nature communications · 2015Article
- A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies.Frontiers in neural circuits · 2015Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Targeted transgene integration overcomes variability of position effects in zebrafish.Development (Cambridge, England) · 2014Article
- New model systems to illuminate thyroid organogenesis. Part I: an update on the zebrafish toolbox.European thyroid journal · 2013Review
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- Attachment site recognition and regulation of directionality by the serine integrases.Nucleic acids research · 2013Article
- Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Site-specific recombinases (SSRs) are powerful tools for genome manipulation, used in diverse organisms including Drosophila melanogaster, mouse, Arabidopsis, zebrafish, and human cultured cells. The integrase from the bacteriophage ΦC31 belongs to the large serine family of integrases, and in contrast to other widely used SSRs such as Cre and Flp, recombination is directional and therefore irreversible. We have developed a vector system for recombinase-mediated cassette exchange (RMCE) in the zebrafish, allowing swapping of the coding sequence in an integrated transgene. Utilizing codon-optimized ΦC31 integrase RNA bearing the 3'UTR from the nanos1 gene, we replaced the egfp coding sequence of an integrated reporter transgene with mCherry coding sequence. Recombination was achieved at high efficiency in both somatic cells and in the germline. We demonstrate an effective approach to RMCE, increasing the repertoire of tools available to manipulate the zebrafish genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.