ArticleMethods in molecular biology (Clifton, N.J.)2011
Creating a "hopeful monster": mouse forward genetic screens.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- Secretory pathway calcium ATPase 1 (SPCA1) controls mouse neural tube closure by regulating cytoskeletal dynamics.Development (Cambridge, England) · 2018Article
- Detection of functional protein domains by unbiased genome-wide forward genetic screening.Scientific reports · 2018Article
- Article
- rahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline.PLoS genetics · 2017Article
- After GWAS: mice to the rescue?Current opinion in immunology · 2012Review
- Multiplex Chromosomal Exome Sequencing Accelerates Identification of ENU-Induced Mutations in the Mouse.G3 (Bethesda, Md.) · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
One of the most straightforward approaches to making novel biological discoveries is the forward genetic screen. The time is ripe for forward genetic screens in the mouse since the mouse genome is sequenced, but the function of many of the genes remains unknown. Today, with careful planning, such screens are within the reach of even small individual labs. In this chapter we first discuss the types of screens in existence, as well as how to design a screen to recover mutations that are relevant to the interests of a lab. We then describe how to create mutations using the chemical N-ethyl-N-nitrosourea (ENU), including a detailed injection protocol. Next, we outline breeding schemes to establish mutant lines for each type of screen. Finally, we explain how to map mutations using recombination and how to ensure that a particular mutation causes a phenotype. Our goal is to make forward genetics in the mouse accessible to any lab with the desire to do it.
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Registered trials
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