ArticleMethods in molecular biology (Clifton, N.J.)2011
Ex utero culture and live imaging of mouse embryos.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm.Developmental cell · 2023Article
- Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools.Nature communications · 2023Article
- Elevated homocysteine activates unfolded protein responses and causes aberrant trophoblast differentiation and mouse blastocyst development.Physiological reports · 2022Article
- Rapid and efficient degradation of endogenous proteins in vivo identifies stage-specific roles of RNA Pol II pausing in mammalian development.Developmental cell · 2022Article
- Mouse embryo phenotyping with optical coherence tomography.Frontiers in cell and developmental biology · 2022Review
- WholemountBio-protocol · 2021Article
- Bioengineering in vitro models of embryonic development.Stem cell reports · 2021Review
- Embryonic Mouse Cardiodynamic OCT Imaging.Journal of cardiovascular development and disease · 2020Review
- Staging mouse preimplantation development in vivo using optical coherence microscopy.Journal of biophotonics · 2019Article
- Adaptation of a Mice Doppler Echocardiography Platform to Measure Cardiac Flow Velocities for Embryonic Chicken and Adult Zebrafish.Frontiers in bioengineering and biotechnology · 2019Article
- Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice.Endocrinology · 2018Article
- GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.Developmental cell · 2014Article
- A method to investigate radial glia cell behavior using two-photon time-lapse microscopy in an ex vivo model of spinal cord development.Frontiers in neuroanatomy · 2014Article
- Congenital heart malformations induced by hemodynamic altering surgical interventions.Frontiers in physiology · 2014Review
- FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse.Development (Cambridge, England) · 2013Article
- Hierarchical approaches for systems modeling in cardiac development.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Mouse genetic approaches when combined with live imaging tools have the potential to revolutionize our current understanding of mammalian biology. The availability and improvement of a wide variety of fluorescent proteins have provided indispensable tools to visualize cells in living organisms. It is now possible to generate genetically modified mouse strains expressing fluorescent proteins in a tissue-specific manner. These reporter-expressing strains make it possible to image dynamic cell behaviors in the context of a living embryo. Since mouse embryos develop within the uterus, live imaging experiments require culture conditions that closely mimic those in vivo. Over the past few decades, significant advances have been made in developing conditions for culturing both pre- and postimplantation stage embryos. In this chapter, we will discuss methods for ex utero culture of preimplantation and postimplantation stage mouse embryos. In particular, we will describe protocols for collecting embryos at various stages, setting up culture conditions for imaging and using laser scanning confocal microscopy to visualize live processes in mouse embryos expressing fluorescent reporters.
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Registered trials
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