ArticlePlanta2012
Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman-Birk protease inhibitor in seeds.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
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- Elemental sulfur concentration can be used as a rapid, reliable, and cost-effective predictor of sulfur amino acid content of soybean seeds.Scientific reports · 2024Article
- Lead Toxicity-Mediated Growth and Metabolic Alterations at Early Seedling Stages of Maize (Plants (Basel, Switzerland) · 2023Article
- Genetic and molecular understanding for the development of methionine-rich maize: a holistic approach.Frontiers in plant science · 2023Review
- Sulfur in Seeds: An Overview.Plants (Basel, Switzerland) · 2022Review
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Increased sulfur-containing amino acid content and altered conformational characteristics of soybean proteins by rebalancing 11S and 7S compositions.Frontiers in plant science · 2022Article
- Biofortification of Cereals and Pulses Using New Breeding Techniques: Current and Future Perspectives.Frontiers in nutrition · 2021Review
- Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds.Scientific reports · 2020Article
- The Level of Methionine Residues in Storage Proteins Is the Main Limiting Factor of Protein-Bound-Methionine Accumulation in Arabidopsis Seeds.Frontiers in plant science · 2020Article
- Common Bean (Plants (Basel, Switzerland) · 2019Article
- Impact of overexpression of cytosolic isoform of O-acetylserine sulfhydrylase on soybean nodulation and nodule metabolome.Scientific reports · 2018Article
- Biofortified Crops Generated by Breeding, Agronomy, and Transgenic Approaches Are Improving Lives of Millions of People around the World.Frontiers in nutrition · 2018Review
- Article
- Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition.Frontiers in plant science · 2015Article
- Effects of proteome rebalancing and sulfur nutrition on the accumulation of methionine rich δ-zein in transgenic soybeans.Frontiers in plant science · 2014Article
- Structure of soybean serine acetyltransferase and formation of the cysteine regulatory complex as a molecular chaperone.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino acids through the expression of heterologous proteins have met with limited success. Here, we report a successful strategy to increase the cysteine content of soybean seed through the overexpression of a key sulfur assimilatory enzyme. We have generated several transgenic soybean plants that overexpress a cytosolic isoform of O-acetylserine sulfhydrylase (OASS). These transgenic soybean plants exhibit a four- to tenfold increase in OASS activity when compared with non-transformed wild-type. The OASS activity in the transgenic soybeans was significantly higher at all the stages of seed development. Unlike the non-transformed soybean plants, there was no marked decrease in the OASS activity even at later stages of seed development. Overexpression of cytosolic OASS resulted in a 58-74% increase in protein-bound cysteine levels compared with non-transformed wild-type soybean seeds. A 22-32% increase in the free cysteine levels was also observed in transgenic soybeans overexpressing OASS. Furthermore, these transgenic soybean plants showed a marked increase in the accumulation of Bowman-Birk protease inhibitor, a cysteine-rich protein. The overall increase in soybean total cysteine content (both free and protein-bound) satisfies the recommended levels required for the optimal growth of monogastric animals.
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