ArticleMolecular and cellular biochemistry2012
The conditioned medium from a stable human GDF3-expressing CHO cell line, induces the differentiation of PC12 cells.
Article in Molecular and cellular biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 14 citations in OpenAlex.
- Clinicopathological relevance of stem cell marker growth and differentiation factor 3 in esophageal squamous cell carcinoma.Exploration of targeted anti-tumor therapy · 2023Article
- Long-term arsenic exposure impairs differentiation in mouse embryonal stem cells.Journal of applied toxicology : JAT · 2021Article
- Network-driven plasma proteomics expose molecular changes in the Alzheimer's brain.Molecular neurodegeneration · 2016Article
- Wnt11 Gene Therapy with Adeno-associated Virus 9 Improves Recovery from Myocardial Infarction by Modulating the Inflammatory Response.Scientific reports · 2016Article
- Production, Isolation, and Structural Analysis of Ligands and Receptors of the TGF-β Superfamily.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Article
- GDF3 inhibits the growth of breast cancer cells and promotes the apoptosis induced by Taxol.Journal of cancer research and clinical oncology · 2012Article
- SNIP1: a new activator of HSE signaling pathway.Molecular and cellular biochemistry · 2012Article
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9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the transforming growth factor-β (TGF-β) superfamily have significant roles in the regulation of a wide variety of physiological processes. In our present work, phylogenetic tree analysis showed that human GDF3 (Growth and differentiation factor 3) and human GDF1 formed a subgroup of closely related molecules. Through quantitative real-time PCR analysis in different human tissues, GDF1 and GDF3 expression level had a big difference in brain. GDF3 could activate downstream signaling through associating with ALK7 (Activin receptor-like kinase 7) in a Cripto-dependent fashion. A CHO cell line stably transfected with the encoding sequence of GDF3, named CHO-GDF3, was established. Western blotting analysis demonstrated that GDF3 protein could be secreted into the medium from CHO cells and immunofluorescence experiment showed that GDF3 was mainly distributed in cytoplasm of the stable cell line, the primary hippocampal neurons, and brain tissues. Furthermore, the conditioned medium from CHO-GDF3 could reduce PC12 cell growth and induce cell differentiation. All these findings bring new insights into the functional study of GDF3.
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