ArticlePloS one2011
Analysis of imprinted gene expression in normal fertilized and uniparental preimplantation porcine embryos.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- A comprehensive genome-wide scan for parent-of-origin expressed genes in the pig clarifies the conservation landscape of genomic imprinting.Genetics, selection, evolution : GSE · 2026Article
- Review
- Short Communication: The imprinted and paternally expressed MIMT1 lncRNA ortholog in pigs.Journal of animal science · 2025Article
- Differential Expression of RNAseq Imprinted Genes from Bovine Females Before and After Puberty.Biochemical genetics · 2023Article
- The AIRN lncRNA is imprinted and paternally expressed in pigs.Journal of animal science · 2023Article
- The single-cell expression profile of transposable elements and transcription factors in human early biparental and uniparental embryonic development.Frontiers in cell and developmental biology · 2022Article
- Loss of Monoallelic Expression ofFrontiers in genetics · 2022Article
- Mouse oocyte vitrification with and without dimethyl sulfoxide: influence on cryo-survival, development, and maternal imprinted gene expression.Journal of assisted reproduction and genetics · 2021Article
- Genome-wide identification of imprinted genes in pigs and their different imprinting status compared with other mammals.Zoological research · 2020Article
- The Landscape of Genomic Imprinting at the PorcineG3 (Bethesda, Md.) · 2020Article
- Article
- The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos.Genome biology · 2020Article
- No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas.Cellular and molecular life sciences : CMLS · 2019Article
- Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs.International journal of molecular sciences · 2016Article
- mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons with Humans, Mice and Pigs.Scientific reports · 2015Article
- Overexpression of OCT4A ortholog elevates endogenous XIST in porcine parthenogenic blastocysts.The Journal of reproduction and development · 2015Article
- Karyotype characterization of in vivo- and in vitro-derived porcine parthenogenetic cell lines.PloS one · 2014Article
- Inhibition of histone deacetylases enhances DNA damage repair in SCNT embryos.Cell cycle (Georgetown, Tex.) · 2014Article
- Genome wide screening of candidate genes for improving piglet birth weight using high and low estimated breeding value populations.International journal of biological sciences · 2014Article
- The putative promoters of germ cell-specific genes and Nanog are hypomethylated in chicken sperm.The Journal of reproduction and development · 2014Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study quantitative real-time PCR was used to determine the expression status of eight imprinted genes (GRB10, H19, IGF2R, XIST, IGF2, NNAT, PEG1 and PEG10) during preimplantation development, in normal fertilized and uniparental porcine embryos. The results demonstrated that, in all observed embryo samples, a non imprinted gene expression pattern up to the 16-cell stage of development was common for most genes. This was true for all classes of embryo, regardless of parental-origins and the direction of imprint. However, several differentially expressed genes (H19, IGF2, XIST and PEG10) were detected amongst the classes at the blastocyst stage of development. Most interestingly and despite the fact that maternally and paternally expressed genes should not be expressed in androgenones and parthenogenones, respectively, both uniparental embryos expressed these genes when tested for in this study. In order to account for this phenomenon, we compared the expression patterns of eight imprinted genes along with the methylation status of the IGF2/H19 DMR3 in haploid and diploid parthenogenetic embryos. Our findings revealed that IGF2, NNAT and PEG10 were silenced in haploid but not diploid parthenogenetic blastocysts and differential methylation of the IGF2/H19 DMR3 was consistently observed between haploid and diploid parthenogenetic blastocysts. These results appear to suggest that there exists a process to adjust the expression status of imprinted genes in diploid parthenogenetic embryos and that this phenomenon may be associated with altered methylation at an imprinting control region. In addition we believe that imprinted expression occurs in at least four genes, namely H19, IGF2, XIST and PEG10 in porcine blastocyst stage embryos.
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