ArticleJournal of bacteriology2011
Glycosylation of DsbA in Francisella tularensis subsp. tularensis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Designing of a conserved subunit multiepitope vaccine candidate against Francisella tularensis Schu S4 using immunoinformatics.Scientific reports · 2026Article
- Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.Scientific reports · 2020Article
- The Francisella tularensis Polysaccharides: What Is the Real Capsule?Microbiology and molecular biology reviews : MMBR · 2020Review
- Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide.Scientific reports · 2019Article
- Challenges of Francisella classification exemplified by an atypical clinical isolate.Diagnostic microbiology and infectious disease · 2018Article
- Further Characterization of the Capsule-Like Complex (CLC) Produced byFrontiers in cellular and infection microbiology · 2018Article
- Whole-Genome Relationships among Francisella Bacteria of Diverse Origins Define New Species and Provide Specific Regions for Detection.Applied and environmental microbiology · 2017Article
- Role of Glycosylation/Deglycolysation Processes inFrontiers in cellular and infection microbiology · 2017Review
- Glycosylation of a Capsule-Like Complex (CLC) byFrontiers in microbiology · 2017Article
- The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of theFrontiers in cellular and infection microbiology · 2017Article
- Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein.Virulence · 2016Article
- FipB, an essential virulence factor of Francisella tularensis subsp. tularensis, has dual roles in disulfide bond formation.Journal of bacteriology · 2014Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- Comparative review of Francisella tularensis and Francisella novicida.Frontiers in cellular and infection microbiology · 2014Review
- In vitro activity of Neisseria meningitidis PglL O-oligosaccharyltransferase with diverse synthetic lipid donors and a UDP-activated sugar.The Journal of biological chemistry · 2013Article
- Roles forPathogens (Basel, Switzerland) · 2012Article
- Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence.Molecular & cellular proteomics : MCP · 2012Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Francisella tularensis subsp. tularensis, DsbA has been shown to be an essential virulence factor and has been observed to migrate to multiple protein spots on two-dimensional electrophoresis gels. In this work, we show that the protein is modified with a 1,156-Da glycan moiety in O-linkage. The results of mass spectrometry studies suggest that the glycan is a hexasaccharide, comprised of N-acetylhexosamines, hexoses, and an unknown monosaccharide. Disruption of two genes within the FTT0789-FTT0800 putative polysaccharide locus, including a galE homologue (FTT0791) and a putative glycosyltransferase (FTT0798), resulted in loss of glycan modification of DsbA. The F. tularensis subsp. tularensis ΔFTT0798 and ΔFTT0791::Cm mutants remained virulent in the murine model of subcutaneous tularemia. This indicates that glycosylation of DsbA does not play a major role in virulence under these conditions. This is the first report of the detailed characterization of the DsbA glycan and putative role of the FTT0789-FTT0800 gene cluster in glycan biosynthesis.
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Registered trials
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