ArticleApplied and environmental microbiology2011
Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for the detection of Salmonella spp. in produce.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Application and evaluation of digital PCR platforms for same-day detection ofCurrent research in food science · 2026Article
- Loop-Mediated Isothermal Amplification Assay for Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection.Nature communications · 2025Article
- Development of a Novel Nested-RT-LAMP Assay for the Rapid and Accurate Coronavirus Disease-2019 Diagnosis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2025Article
- Viral Involvement in Oral Potentially Malignant Disorders: A Scoping Review.Cancer management and research · 2025Review
- Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP.Applied microbiology and biotechnology · 2024Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Article
- Salmonella detection with LAMP and qPCR and identification of serovars of interest by multiplex qPCR in poultry carcasses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Microbial Inoculants as Plant Biostimulants: A Review on Risk Status.Life (Basel, Switzerland) · 2022Review
- Article
- RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19.Nature protocols · 2021Article
- COVID-19: Current Trends in Invitro Diagnostics.Indian journal of clinical biochemistry : IJCB · 2020Review
- Microfluidic droplet application for bacterial surveillance in fresh-cut produce wash waters.PloS one · 2020Article
- Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform.Sensors (Basel, Switzerland) · 2019Article
- Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions.Foodborne pathogens and disease · 2018Review
- Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.Science immunology · 2016Article
- Loop-Mediated Isothermal Amplification (LAMP) for Rapid Detection and Quantification of Dehalococcoides Biomarker Genes in Commercial Reductive Dechlorinating Cultures KB-1 and SDC-9.Applied and environmental microbiology · 2016Article
- Evaluation of Two Loop-mediated Isothermal Amplification Methods for the Detection ofItalian journal of food safety · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contamination of foods, especially produce, with Salmonella spp. is a major concern for public health. Several methods are available for the detection of Salmonella in produce, but their relative efficiency for detecting Salmonella in commonly consumed vegetables, often associated with outbreaks of food poisoning, needs to be confirmed. In this study, the effectiveness of three molecular methods for detection of Salmonella in six produce matrices was evaluated and compared to the FDA microbiological detection method. Samples of cilantro (coriander leaves), lettuce, parsley, spinach, tomato, and jalapeno pepper were inoculated with Salmonella serovars at two different levels (10(5) and <10(1) CFU/25 g of produce). The inoculated produce was assayed by the FDA Salmonella culture method (Bacteriological Analytical Manual) and by three molecular methods: quantitative real-time PCR (qPCR), quantitative reverse transcriptase real-time PCR (RT-qPCR), and loop-mediated isothermal amplification (LAMP). Comparable results were obtained by these four methods, which all detected as little as 2 CFU of Salmonella cells/25 g of produce. All control samples (not inoculated) were negative by the four methods. RT-qPCR detects only live Salmonella cells, obviating the danger of false-positive results from nonviable cells. False negatives (inhibition of either qPCR or RT-qPCR) were avoided by the use of either a DNA or an RNA amplification internal control (IAC). Compared to the conventional culture method, the qPCR, RT-qPCR, and LAMP assays allowed faster and equally accurate detection of Salmonella spp. in six high-risk produce commodities.
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