ArticleApplied and environmental microbiology2011
Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed.
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- A Multi-Tetracycline Responsive Induction System for Gene Expression inbioRxiv : the preprint server for biology · 2025Article
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- Toward Antibody Production in Genome-MinimizedACS synthetic biology · 2025Article
- Recombinant Production of Bovine αMicroorganisms · 2025Article
- Microbial flora of the respiratory tract and skin of artisanal municipal solid waste handlers in Aba, Abia State, Nigeria.Access microbiology · 2025Article
- Enhancing High-Level Food-Grade Expression of Glutamate Decarboxylase and Its Application in the Production of γ-Aminobutyric Acid.Journal of microbiology and biotechnology · 2024Article
- The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes.Microbial cell factories · 2024Article
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- Advances in recombinant protease production: current state and perspectives.World journal of microbiology & biotechnology · 2024Review
- Creating Single-Cell Protein-ProducingScientifica · 2024Article
- Let There Be Light: Genome Reduction EnablesACS synthetic biology · 2023Article
- Metabolic engineering of Bacillus subtilis toward the efficient and stable production of CAMB Express · 2023Article
- A biostatistical approach for augmenting rare bianthraquinone antibiotic production by Streptomyces sp. RA-WS2 using Taguchi design.AMB Express · 2022Article
- A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.Bioresources and bioprocessing · 2022Article
- An operator-based expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A novel technically compliant expression system was developed for heterologous protein production in Bacillus subtilis with the aim of increasing product yields at the same time as decreasing production costs. Standard systems involve the positively regulated manP promoter of the mannose operon, which led to relatively high product yields of 5.3% (5.3 g enhanced green fluorescent protein [eGFP] per 100 g cell dry weight [CDW]) but required large quantities of mannose to induce the reactions, thus rendering the system's technical application rather expensive. To improve this situation, mutant B. subtilis strains were used: the ΔmanA (mannose metabolism) strain TQ281 and the ΔmanP (mannose uptake) strain TQ356. The total amount of inducer could be reduced with TQ281, which, however, displayed sensitivity to mannose. An inducer-independent self-induction system was developed with TQ356 to further improve the cost efficiency and product yield of the system, in which glucose prevents induction by carbon catabolite repression. To create optimal self-induction conditions, a glucose-limited process strategy, namely, a fed-batch process, was utilized as follows. The initiation of self-induction at the beginning of the glucose-restricted transition phase between the batch and fed-batch phase of fermentation and its maintenance throughout the glucose-limiting fed-batch phase led to a nearly 3-fold increase of product yield, to 14.6%. The novel B. subtilis self-induction system thus makes a considerable contribution to improving product yield and reducing the costs associated with its technical application.
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