ArticleAnalytical biochemistry2011
Optimization of monosaccharide determination using anthranilic acid and 1-phenyl-3-methyl-5-pyrazolone for gastropod analysis.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- A comprehensive review on structural, chemical, and functional perspectives of dietary polysaccharide-driven gut microbiota modulation: Mechanisms and challenges.Biochemistry and biophysics reports · 2026Review
- Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (Foods (Basel, Switzerland) · 2024Article
- Cloning, expression and characterisation of a novel mollusc α-1,2-Fucosyltransferase from Crassostrea gigas (CgFUT2).Glycoconjugate journal · 2024Article
- Genus-wide analysis ofApplied and environmental microbiology · 2024Article
- Determination, expression and characterization of an UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I (GnT-I) from the Pacific oyster, Crassostrea gigas.Glycoconjugate journal · 2024Article
- Expression and Characterization of a β-Galactosidase from the Pacific Oyster,International journal of molecular sciences · 2023Article
- Determination of the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent.The protein journal · 2019Article
- Liquid Chromatography-Tandem Mass Spectrometry Approach for Determining Glycosidic Linkages.Analytical chemistry · 2018Article
- Effective extraction of Arabidopsis adherent seed mucilage by ultrasonic treatment.Scientific reports · 2017Article
- Monosaccharide profiling of silkworm (Bombyx mori L.) nervous system during development and aging.Invertebrate neuroscience : IN · 2016Article
- A novel mass spectrometric strategy "BEMAP" reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli.Scientific reports · 2016Article
- Glycomics: revealing the dynamic ecology and evolution of sugar molecules.Journal of proteomics · 2016Review
- Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities.Journal of food science and technology · 2016Article
- Characterization of plants expressing the human β1,4-galactosyltrasferase gene.Plant physiology and biochemistry : PPB · 2015Article
- Mucin-Type O-Glycosylation in Invertebrates.Molecules (Basel, Switzerland) · 2015Review
- Biosynthesis of the fungal cell wall polysaccharide galactomannan requires intraluminal GDP-mannose.The Journal of biological chemistry · 2012Article
- Methylation--an uncommon modification of glycans.Biological chemistry · 2012Review
- O-Glycosylation of snails.Glycoconjugate journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The protein-linked glycomes and, thereby, the range of individual monosaccharides of invertebrates differ from those of mammals due to a number of special modifications; therefore, it is necessary to adapt methods for monosaccharide analysis in order to cover these. We optimized the labeling procedure for anthranilic acid (AA) and 1-phenyl-3-methyl-5-pyrazolone (PMP) and the subsequent separation of the labeled monosaccharides on high-performance liquid chromatography (HPLC), with the result that we were able to identify 26 different monosaccharides. The detection limit for anthranilic acid derivatives obtained was 65 fmol, and a reliable quantification of samples was possible up to 200 nmol under the tested conditions. PMP derivatives showed a significantly higher detection limit but allow quantification of larger sample amounts. Applying these methods on snails, their impressive set of monosaccharide constituents, including methylated sugars, was shown.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.