ArticlePhytochemistry2011
A virus-induced gene silencing approach to understanding alkaloid metabolism in Catharanthus roseus.
Article in Phytochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 136 citations in OpenAlex.
- Discovery of iridoid cyclase completes the iridoid pathway in asterids.Nature plants · 2025Article
- Identification of DELLA and GID1 genes in Catharanthus roseus and their potential role in regulating vindoline biosynthesis.Plant molecular biology · 2025Article
- Transcriptional Reprogramming Deploys a Compartmentalized 'Timebomb' in Catharanthus roseus to Fend Off Chewing Herbivores.Plant, cell & environment · 2025Article
- A long road ahead to reliable and complete medicinal plant genomes.Nature communications · 2025Review
- Critical parameters for robustPlant direct · 2024Article
- The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus.Plant cell reports · 2024Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants.Plant methods · 2024Article
- Single-cell multi-omics in the medicinal plant Catharanthus roseus.Nature chemical biology · 2023Article
- Vinca alkaloids as a potential cancer therapeutics: recent update and future challenges.3 Biotech · 2023Review
- Article
- New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach.Plants (Basel, Switzerland) · 2023Article
- In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria).Planta · 2022Article
- Ocimum sanctum, OscWRKY1, regulates phenylpropanoid pathway genes and promotes resistance to pathogen infection in Arabidopsis.Plant molecular biology · 2022Article
- Understanding the possible influence ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- A Rapid and Efficient Vacuum-Based Agroinfiltration Protocol for Transient Gene Overexpression in Leaves of Catharanthus roseus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Generating an EMS Mutant Population and Rapid Mutant Screening by Thin-Layer Chromatography Enables the Studies of Monoterpenoid Indole Alkaloids Biosynthesis in Catharanthus Roseus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Terpenoid indole alkaloid biosynthesis in Catharanthus roseus: effects and prospects of environmental factors in metabolic engineering.Biotechnology letters · 2021Review
- Improved virus-induced gene silencing allows discovery of a serpentine synthase gene in Catharanthus roseus.Plant physiology · 2021Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The anticancer agents vinblastine and vincristine are bisindole alkaloids derived from coupling vindoline and catharanthine, monoterpenoid indole alkaloids produced exclusively by the Madagascar periwinkle (Catharanthus roseus). Industrial production of vinblastine and vincristine currently relies on isolation from C. roseus leaves, a process that affords these compounds in 0.0003-0.01% yields. Metabolic engineering efforts to either improve alkaloid content or provide alternative sources of the bisindole alkaloids ultimately rely on the isolation and characterization of the genes involved. Several vindoline biosynthetic genes have been isolated, and the cellular and subcellular organization of the corresponding enzymes has been well studied. However, due to the leaf-specific localization of vindoline biosynthesis, and the lack of production of this precursor in cell suspension and hairy root cultures of C. roseus, further elucidation of this pathway demands the development of reverse genetics approaches to assay gene function in planta. The bipartite pTRV vector system is a Tobacco Rattle Virus-based virus-induced gene silencing (VIGS) platform that has provided efficient and effective means to assay gene function in diverse plant systems. A VIGS method was developed herein to investigate gene function in C. roseus plants using the pTRV vector system. The utility of this approach in understanding gene function in C. roseus leaves is demonstrated by silencing known vindoline biosynthetic genes previously characterized in vitro.
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