Evidence map›Paper›PMID 21799909›Full record

ArticlePloS one2011

Expression and rhythmic modulation of circulating microRNAs targeting the clock gene Bmal1 in mice.

Vikram R Shende, Marianna M Goldrick, Suchitra Ramani, David J Earnest

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 118 citations in OpenAlex.

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  7. Chrono-Nutrition: Circadian Rhythm and Personalized Nutrition.International journal of molecular sciences · 2023
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1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Vikram R ShendeDepartment of Biology, Texas A&M University, College Station, Texas, United States of America.
Marianna M Goldrick
Suchitra Ramani
David J Earnest
Bioo Scientific (United States) · USTexas A&M University · US

Funding

The Circadian Clock in the Context of the Cyanobacterial CellP01NS039546 · NINDS · TEXAS A&M UNIVERSITY SYSTEM · PI THOMAS, TERRY L · 2000 to 2010
$10.6M
NINDS NIH HHS P01 NS039546NINDS NIH HHS P01 NS39546
6 · The paper itself

Abstract

MicroRNAs (miRNAs) interact with 3' untranslated region (UTR) elements of target genes to regulate mRNA stability or translation and thus play a role in regulating many different biological processes, including circadian rhythms. However, specific miRNAs mediating the regulation of essential clock genes remain largely unknown. Because vesicles containing membrane-bound miRNAs are present in the circulatory system, we examined miRNAs predicted to target the clock gene, Bmal1, for evidence of rhythmic fluctuations in circulating levels and modulatory effects on the 3' UTR activity of Bmal1. A number of miRNAs with Bmal1 as a predicted target were expressed in the serum of mice exposed to LD 12:12 and of these miRNAs, miR-152 and miR-494 but not miR-142-3p were marked by diurnal oscillations with bimodal peaks in expression occurring near the middle of the day and 8 or 12 hr later during the night. Co-transfection of pre-miR over-expression constructs for miR-494 and miR-142-3p in HEK293 cells had significant effects in repressing luciferase-reported Bmal1 3' UTR activity by as much as 60%, suggesting that these miRNAs may function as post-transcriptional modulators of Bmal1. In conjunction with previous studies implicating miRNAs as extracellular regulatory signals, our results suggest that circulating miRNAs may play a role in the regulation of the molecular clockworks in peripheral circadian oscillators.

Indexed as

3' Untranslated RegionsAnimalsARNTL Transcription FactorsCircadian ClocksGene Expression RegulationHEK293 CellsHumansMaleMiceMice, Inbred BALB CMicroRNAsTime Factors3' Untranslated RegionsARNTL Transcription FactorsMicroRNAs

Identifiers

PMID21799909
PMCPMC3142187
OpenAlexW2072400208

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.