ArticlePloS one2011
Identification of Toxoplasma gondii cAMP dependent protein kinase and its role in the tachyzoite growth.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Purine nucleosides replace cAMP in allosteric regulation of PKA in trypanosomatid pathogens.eLife · 2024Article
- FromiScience · 2024Article
- Probing ligand selectivity in pathogens.eLife · 2023Article
- Protein kinases on carbon metabolism: potential targets for alternative chemotherapies against toxoplasmosis.Frontiers in cellular and infection microbiology · 2023Review
- Article
- Exposure to dexamethasone modifies transcriptomic responses of free-living stages of Strongyloides stercoralis.PloS one · 2021Article
- Uncovering the Cyclic AMP Signaling Pathway of the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2020Article
- Druggable Targets in Cyclic Nucleotide Signaling Pathways in Apicomplexan Parasites and Kinetoplastids against Disabling Protozoan Diseases in Humans.International journal of molecular sciences · 2019Review
- Crosstalk between PKA and PKG controls pH-dependent host cell egress ofThe EMBO journal · 2017Article
- Suppressors for Human Epidermal Growth Factor Receptor 2/4 (HER2/4): A New Family of Anti-Toxoplasmic Agents in ARPE-19 Cells.The Korean journal of parasitology · 2017Article
- PKA and Apicomplexan Parasite Diseases.Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme · 2017Review
- Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog.Parasites & vectors · 2016Article
- Article
- The central role of cAMP in regulating Plasmodium falciparum merozoite invasion of human erythrocytes.PLoS pathogens · 2014Article
- Dual transcriptional profiling of mice and Toxoplasma gondii during acute and chronic infection.BMC genomics · 2014Article
- Identification of mutations in TgMAPK1 of Toxoplasma gondii conferring resistance to 1NM-PP1.International journal for parasitology. Drugs and drug resistance · 2013Article
- DNA prime and peptide boost immunization protocol encoding the Toxoplasma gondii GRA4 induces strong protective immunity in BALB/c mice.BMC infectious diseases · 2013Article
- Protein kinases of Toxoplasma gondii: functions and drug targets.Parasitology research · 2013Review
- Anti-Iranian journal of parasitologyArticle
- The immunomodulatory effects of rolipram abolish drug-resistant latent phase ofJournal of microscopy and ultrastructureArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundcAMP-dependent protein kinase (PKA) has been implicated in the asexual stage of the Toxoplasma gondii life cycle through assaying the effect of a PKA-specific inhibitor on its growth rate. Since inhibition of the host cell PKA cannot be ruled out, a more precise evaluation of the role of PKA, as well as characterization of the kinase itself, is necessary. METHODOLOGY/PRINCIPAL FINDING: The inhibitory effects of two PKA inhibitors, H89, an ATP-competitive chemical inhibitor, and PKI, a substrate-competitive mammalian natural peptide inhibitor, were estimated. In the in vitro kinase assay, the inhibitory effect of PKI on a recombinant T. gondii PKA catalytic subunit (TgPKA-C) was weaker compared to that on mammalian PKA-C. In a tachyzoite growth assay, PKI had little effect on the growth of tachyzoites, whereas H89 strongly inhibited it. Moreover, T. gondii PKA regulatory subunit (TgPKA-R)-overexpressing tachyzoites showed a significant growth defect. CONCLUSIONS/SIGNIFICANCE: Our data suggest that PKA plays an important role in the growth of tachyzoites, and the inhibitory effect of substrate-competitive inhibitor PKI on T. gondii PKA was low compared to that of the ATP competitive inhibitor H89.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.