ArticlePloS one2011
A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Interplay between the Xer recombination system and the dissemination of antibioresistance in Acinetobacter baumannii.Nucleic acids research · 2025Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Bacterial cell proliferation: from molecules to cells.FEMS microbiology reviews · 2021Review
- Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome.PLoS genetics · 2018Article
- Division-induced DNA double strand breaks in the chromosome terminus region of Escherichia coli lacking RecBCD DNA repair enzyme.PLoS genetics · 2017Article
- Fast growth conditions uncouple the final stages of chromosome segregation and cell division in Escherichia coli.PLoS genetics · 2017Article
- Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli.PLoS genetics · 2017Article
- How Xer-exploiting mobile elements overcome cellular control.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Defining the rate-limiting processes of bacterial cytokinesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Brucella abortus Cell Cycle and Infection Are Coordinated.Trends in microbiology · 2015Review
- Spatial coordination between chromosomes and cell division proteins in Escherichia coli.Frontiers in microbiology · 2015Article
- Cell cycle regulation by the bacterial nucleoid.Current opinion in microbiology · 2014Review
- Role of RNA polymerase and transcription in the organization of the bacterial nucleoid.Chemical reviews · 2013Review
- FtsK actively segregates sister chromosomes in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Sister chromatid interactions in bacteria revealed by a site-specific recombination assay.The EMBO journal · 2012Article
- Molecular motors for DNA translocation in prokaryotes.Current opinion in biotechnology · 2012Review
- Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif.Nucleic acids research · 2012Article
- Single-molecule imaging of DNA curtains reveals mechanisms of KOPS sequence targeting by the DNA translocase FtsK.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe FtsK DNA-translocase controls the last steps of chromosome segregation in E. coli. It translocates sister chromosomes using the KOPS DNA motifs to orient its activity, and controls the resolution of dimeric forms of sister chromosomes by XerCD-mediated recombination at the dif site and their decatenation by TopoIV. METHODOLOGY: We have used XerCD/dif recombination as a genetic trap to probe the interaction of FtsK with loci located in different regions of the chromosome. This assay revealed that the activity of FtsK is restricted to a ∼400 kb terminal region of the chromosome around the natural position of the dif site. Preferential interaction with this region required the tethering of FtsK to the division septum via its N-terminal domain as well as its translocation activity. However, the KOPS-recognition activity of FtsK was not required. Displacement of replication termination outside the FtsK high activity region had no effect on FtsK activity and deletion of a part of this region was not compensated by its extension to neighbouring regions. By observing the fate of fluorescent-tagged loci of the ter region, we found that segregation of the FtsK high activity region is delayed compared to that of its adjacent regions. SIGNIFICANCE: Our results show that a restricted terminal region of the chromosome is specifically dedicated to the last steps of chromosome segregation and to their coupling with cell division by FtsK.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.