ArticlePloS one2011
The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 41 citations in OpenAlex.
- Putative membrane protein Cgl2168 and its variant enhance thermotolerance inSynthetic and systems biotechnology · 2026Article
- The exploitation of nutrient metals by bacteria for survival and infection in the gut.PLoS pathogens · 2025Review
- Regulatory Characterization of Two Cop Systems for Copper Resistance inInternational journal of molecular sciences · 2025Article
- Copper Resistance Mechanism and Copper Response Genes inMicroorganisms · 2024Article
- Metal Messengers: Communication in the Bacterial World through Transition-Metal-Sensing Two-Component Systems.Biochemistry · 2023Review
- A Copper-Responsive Two-Component System Governs Lipoprotein Remodeling in Listeria monocytogenes.Journal of bacteriology · 2023Article
- Copper microenvironments in the human body define patterns of copper adaptation in pathogenic bacteria.PLoS pathogens · 2022Review
- Induction of glutamic acid production by copper in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Article
- Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes.Journal of bacteriology · 2019Article
- Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate.Applied and environmental microbiology · 2019Article
- Continuous Adaptive Evolution of a Fast-GrowingFrontiers in microbiology · 2019Article
- The copper-deprivation stimulon ofThe Journal of biological chemistry · 2018Article
- Copper homeostasis networks in the bacteriumThe Journal of biological chemistry · 2017Article
- Towards the complete proteinaceous regulome ofMicrobial genomics · 2017Article
- An intimate link: two-component signal transduction systems and metal transport systems in bacteria.Future microbiology · 2014Review
- Two-component signal transduction in Corynebacterium glutamicum and other corynebacteria: on the way towards stimuli and targets.Applied microbiology and biotechnology · 2012Review
- Metalloregulation of Gram-positive pathogen physiology.Current opinion in microbiology · 2012Review
- Characterization of the alkaline laccase Ssl1 from Streptomyces sviceus with unusual properties discovered by genome mining.PloS one · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu(2+) was studied using DNA microarrays and revealed 20 genes that showed a ≥ 3-fold increased mRNA level, including cg3281-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. g. a multicopper oxidase (CopO) and a copper-transport ATPase (CopB). In addition, this region includes the copRS genes (previously named cgtRS9) which encode a two-component signal transduction system composed of the histidine kinase CopS and the response regulator CopR. Deletion of the copRS genes increased the sensitivity of C. glutamicum towards copper ions, but not to other heavy metal ions. Using comparative transcriptome analysis of the ΔcopRS mutant and the wild type in combination with electrophoretic mobility shift assays and reporter gene studies the CopR regulon and the DNA-binding motif of CopR were identified. Evidence was obtained that CopR binds only to the intergenic region between cg3285 (copR) and cg3286 in the genome of C. glutamicum and activates expression of the divergently oriented gene clusters cg3285-cg3281 and cg3286-cg3289. Altogether, our data suggest that CopRS is the key regulatory system in C. glutamicum for the extracytoplasmic sensing of elevated copper ion concentrations and for induction of a set of genes capable of diminishing copper stress.
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