ArticlePloS one2011
Automated microinjection of recombinant BCL-X into mouse zygotes enhances embryo development.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 41 citations in OpenAlex.
- Establishment of an integrated automated embryonic manipulation system for producing genetically modified mice.Scientific reports · 2021Article
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Three-Dimensional Autofocusing Visual Feedback for Automated Rare Cells Sorting in Fluorescence Microscopy.Micromachines · 2019Article
- A Review of Automated Microinjection of Zebrafish Embryos.Micromachines · 2018Review
- Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes.Scientific reports · 2016Article
- Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro.Journal of assisted reproduction and genetics · 2014Article
- Dissecting non-coding RNA mechanisms in cellulo by Single-molecule High-Resolution Localization and Counting.Methods (San Diego, Calif.) · 2013Article
- Maternal obesity, infertility and mitochondrial dysfunction: potential mechanisms emerging from mouse model systems.Molecular human reproduction · 2013Review
- Nanofountain probe electroporation (NFP-E) of single cells.Nano letters · 2013Article
- NLRP5 mediates mitochondrial function in mouse oocytes and embryos.Biology of reproduction · 2012Article
- High fat diet induced developmental defects in the mouse: oocyte meiotic aneuploidy and fetal growth retardation/brain defects.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
Progression of fertilized mammalian oocytes through cleavage, blastocyst formation and implantation depends on successful implementation of the developmental program, which becomes established during oogenesis. The identification of ooplasmic factors, which are responsible for successful embryo development, is thus crucial in designing possible molecular therapies for infertility intervention. However, systematic evaluation of molecular targets has been hampered by the lack of techniques for efficient delivery of molecules into embryos. We have developed an automated robotic microinjection system for delivering cell impermeable compounds into preimplantation embryos with a high post-injection survival rate. In this paper, we report the performance of the system on microinjection of mouse embryos. Furthermore, using this system we provide the first evidence that recombinant BCL-XL (recBCL-XL) protein is effective in preventing early embryo arrest imposed by suboptimal culture environment. We demonstrate that microinjection of recBCL-XL protein into early-stage embryos repairs mitochondrial bioenergetics, prevents reactive oxygen species (ROS) accumulation, and enhances preimplantation embryo development. This approach may lead to a possible treatment option for patients with repeated in vitro fertilization (IVF) failure due to poor embryo quality.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.